CRISPR/Cas系统在斑马鱼中的研究进展

doi:10.19586/j.2095-2341.2023.0033

生物技术进展 ›› 2023, Vol. 13 ›› Issue (4): 485-491.DOI: 10.19586/j.2095-2341.2023.0033

• 进展评述 •

CRISPR/Cas系统在斑马鱼中的研究进展

王阿利(), 刘江东()

武汉大学生命科学学院，武汉 430072

收稿日期:2023-03-15 接受日期:2023-03-31 出版日期:2023-07-25 发布日期:2023-08-03
通讯作者: 刘江东
作者简介:王阿利 E-mail: 2020202040077@whu.edu.cn；

Research Progress on the CRISPR/Cas System in Zebrafish

Ali WANG(), Jiangdong LIU()

College of Life Science，Wuhan University，Wuhan 430072，China

Received:2023-03-15 Accepted:2023-03-31 Online:2023-07-25 Published:2023-08-03
Contact: Jiangdong LIU

摘要/Abstract

摘要：

斑马鱼是生物学中十分重要的模式生物，可作为基因功能分析、人类疾病病理学研究和新药研发的有利工具。它具有易于控制操作、与人类进化关系相近的优势，目前已经开发了多种斑马鱼模型用于研究人类相关疾病。聚集的有规则间隔的短回文重复序列及其关联蛋白（the clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins，CRISPR/Cas）技术的出现，大大降低了斑马鱼基因编辑的复杂性。主要描述了CRISPR/Cas系统的基本原理、技术革新，总结了CRISPR/Cas系统在斑马鱼基因敲除或敲入、活细胞成像、转录调控、多重靶向、建立疾病模型中的重要作用，以期为探究CRISPR/Cas系统在斑马鱼基因组学研究中的应用提供一定思路。

关键词: CRISPR/Cas, 斑马鱼, 基因编辑, 基因组学研究, 疾病模型

Abstract:

The zebrafish is an important model organism in biology and a useful tool for gene function analysis， human disease pathology research， and new drug development. It has advantages of easy operation and close evolutionary relationship with humans. Currently， various zebrafish models have been developed for the study of human-related diseases. The emergence of clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins （CRISPR/Cas） technology with regularly spaced short palindromic repeats and their associated proteins has greatly reduced the complexity of zebrafish gene editing. This article mainly described the basic principles and technical innovations of the CRISPR/Cas system， and summarized the important role of the CRISPR/Cas system in zebrafish gene knockout， knock-in， live-cell imaging， transcriptional regulation， multiple targeting， and disease model establishment， with a view to providing a certain idea for exploring the application of the CRISPR/Cas system in zebrafish genomics research.

Key words: CRISPR/Cas, zebrafish, gene editing, genomics research, disease model

中图分类号:

王阿利, 刘江东. CRISPR/Cas系统在斑马鱼中的研究进展[J]. 生物技术进展, 2023, 13(4): 485-491.

Ali WANG, Jiangdong LIU. Research Progress on the CRISPR/Cas System in Zebrafish[J]. Current Biotechnology, 2023, 13(4): 485-491.

图/表 1

参考文献 60

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种类	CRISPR酶	向导RNA	PAM序列	目标序列	应用	参考文献
Ⅱ	Cas9	sgRNA	NGG	DNA	基因组编辑、活细胞成像、转录调控	[9-11]
Ⅴ	Cas12	crRNA/sgRNA	TTN	DNA	基因组编辑	[12-14]
Ⅵ	Cas13	crRNA	—	RNA	RNA编辑和示踪	[15-16]

种类	CRISPR酶	向导RNA	PAM序列	目标序列	应用	参考文献
Ⅱ	Cas9	sgRNA	NGG	DNA	基因组编辑、活细胞成像、转录调控	[9-11]
Ⅴ	Cas12	crRNA/sgRNA	TTN	DNA	基因组编辑	[12-14]
Ⅵ	Cas13	crRNA	—	RNA	RNA编辑和示踪	[15-16]

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CRISPR/Cas系统在斑马鱼中的研究进展

Research Progress on the CRISPR/Cas System in Zebrafish

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