生物技术进展 ›› 2021, Vol. 11 ›› Issue (3): 253-259.DOI: 10.19586/j.2095-2341.2021.0020

• 进展评述 •    下一篇

CRISPR-Cas系统在微生物研究中的应用进展

格日乐其木格1,2,牛振峰2,董丹2,张涛涛2,峥嵘1*   

  1. 1.内蒙古师范大学生命科学与技术学院, 呼和浩特 010022;
    2.北京市农林科学院植物保护环境保护研究所, 北京 100097
  • 收稿日期:2021-02-08 出版日期:2021-05-25 发布日期:2021-03-30
  • 通讯作者: 峥嵘 E-mail:zhengrong09@163.com
  • 作者简介:格日乐其木格 E-mail:1974552627@qq.com
  • 基金资助:
    北京市自然科学基金项目(6192008);北京市农林科学院创新专项(KJCX20200521; KJCX20200110);北京市科技计划项目(D171100007617001)。

Application Progress of CRISPR-Cas System in Microbial Research

GERILEQIMUGE,, NIU Zhenfeng, DONG Dan, ZHANG Taotao, ZHENG Rong   

  1. 1.College of Life Sciences and Technology, Inner Mongolia Normal University, Hohhot 010022, China;
    2.Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
  • Received:2021-02-08 Online:2021-05-25 Published:2021-03-30

摘要: 来源于细菌防御系统的CRISPR-Cas系统因其功能强大、多样性受到广泛关注,也在动物、植物、微生物等各个领域得到了应用并迅速发展。概述了CRISPR-Cas系统的作用机理和分类,并对CRISPR-Cas系统在微生物基因编辑和病毒核酸检测方面的研究及应用进展进行了综述,以期为微生物研究工作提供参考。

关键词: CRISPR-Cas系统, 基因编辑, 核酸检测

Abstract: The CRISPR-Cas system, which is derived from the defense system of bacteria, has attracted wide attention due to its powerful and diverse functions. It has also been applied and developed rapidly in various fields such as animals, plants, and microorganisms. This article summarized the mechanism and classification of the CRISPR-Cas system, and reviewed the research and application progress of the CRISPR-Cas system in microbial gene editing and viral nucleic acid detection, in order to provide references for microbial research.

Key words: CRISPR-Cas system, gene editing, nucleic acid testing