生物技术进展 ›› 2021, Vol. 11 ›› Issue (4): 405-417.DOI: 10.19586/j.2095-2341.2021.0096
• 前沿新技术 • 下一篇
收稿日期:
2021-05-31
接受日期:
2021-06-16
出版日期:
2021-07-25
发布日期:
2021-08-02
作者简介:
林敏 E-mail:linmin@caas.cn
基金资助:
Received:
2021-05-31
Accepted:
2021-06-16
Online:
2021-07-25
Published:
2021-08-02
摘要:
伴随千百年来自然物种进化与人类科技进步,世界农业育种经历了原始育种、传统育种和分子育种三个时代的跨越。生物育种是生物技术育种的简称,属于从转基因育种3.0版跨入智能设计育种4.0版、集各种前沿技术大成的新一代分子育种技术,其中最具代表性的包括培育革命性和颠覆性新品种的全基因组选择、基因编辑和合成生物技术。回顾了国内外农业转基因和生物育种技术的发展历程,分析了我国生物育种面临的严峻挑战,提出了加快我国生物育种技术创新的产业化对策。
中图分类号:
林敏. 农业生物育种技术的发展历程及产业化对策[J]. 生物技术进展, 2021, 11(4): 405-417.
Min LIN. The Development Course and Industrialization Countermeasure of Agricultural Biological Breeding Technology[J]. Current Biotechnology, 2021, 11(4): 405-417.
技术途径 | 作用机制 | 相关产品 |
---|---|---|
植物基因工程 | 减少农药使用和碳排放;免耕增加土壤碳储量;高效利用土地和水资源 | 抗除草剂、抗虫和耐旱节水等转基因作物 |
人工高效固碳途径 | 直接利用二氧化碳合成生物大分子;大幅度增强光合效率;增加碳汇 | 单细胞固碳;C4水稻;人工叶片等 |
人工高效固氮途径 | 克服铵抑制、氧失活等天然固氮体系缺陷;节能节肥;减少碳排放 | 固氮微生物肥料;人工结瘤固氮粮食作物;自主固氮真核生物 |
生物质转化工程 | 将生物质转化为生物炭(biochar)并应用于土壤改良;增加土壤碳储量;生物质饲料化或肥料化 | 生物炭;生物饲料;生物肥料 |
动物基因工程 | 抗重大畜禽疫病;节省饲料;减少药物使用和碳排放 | 节粮高产抗病养殖动物;抗生素替代产品 |
农业细胞工厂 | 节能;高附加值;减少用水量、土地需求和碳排放 | 人造肉汉堡、人造奶冰淇淋等未来合成食品 |
表1 有助于碳减排和碳增汇的生物育种技术及其产品
Table 1 Biological breeding techniques and their products for carbon emission reduction and carbon sink increase
技术途径 | 作用机制 | 相关产品 |
---|---|---|
植物基因工程 | 减少农药使用和碳排放;免耕增加土壤碳储量;高效利用土地和水资源 | 抗除草剂、抗虫和耐旱节水等转基因作物 |
人工高效固碳途径 | 直接利用二氧化碳合成生物大分子;大幅度增强光合效率;增加碳汇 | 单细胞固碳;C4水稻;人工叶片等 |
人工高效固氮途径 | 克服铵抑制、氧失活等天然固氮体系缺陷;节能节肥;减少碳排放 | 固氮微生物肥料;人工结瘤固氮粮食作物;自主固氮真核生物 |
生物质转化工程 | 将生物质转化为生物炭(biochar)并应用于土壤改良;增加土壤碳储量;生物质饲料化或肥料化 | 生物炭;生物饲料;生物肥料 |
动物基因工程 | 抗重大畜禽疫病;节省饲料;减少药物使用和碳排放 | 节粮高产抗病养殖动物;抗生素替代产品 |
农业细胞工厂 | 节能;高附加值;减少用水量、土地需求和碳排放 | 人造肉汉堡、人造奶冰淇淋等未来合成食品 |
1 | VASIL I K. A history of plant biotechnology: from the cell theory of Schleiden and Schwann to biotech crops[J]. Plant Cell Rep., 2008,9:1423-1440. |
2 | DUVICK D N. Biotechnology in the 1930s: the development of hybrid maize[J]. Nat. Rev. Genet., 2001,2(1):69-74. |
3 | KHUSH G S. Green revolution: the way forward[J]. Nat. Rev. Genet., 2001,2(10):815-822. |
4 | VARSHNEY R K, BOHRA A, YU J, et al.. Designing future crops: genomics-assisted breeding comes of age[J]. Trends Plant Sci., 2021,26(6):631-649. |
5 | VAROTTO S, TANI E, ABRAHAM E, et al.. Epigenetics: possible applications in climate-smart crop breeding[J]. J. Exp. Bot., 2020,71(17):5223-5236. |
6 | 林敏. 转基因技术[M]. 北京:中国农业科学技术出版社,2020. |
7 | 农业农村部农业转基因生物安全管理办公室.转基因30年实践[M].北京:中国农业科学技术出版社,2012. |
8 | International Service for the Acquisition of Agri-biotech Applications (ISAAA). Global status of commercialized biotech/GM crops in 2018: biotech crops continue to help meet the challenges of increased population and climate change[R/OL]. ISAAA,2018[2021-06-18]. . |
9 | ZHAO Y S, METTE M F, REIF J C. Genomic selection in hybrid breeding[J]. Plant Breed., 2015, 134(1):1-10. |
10 | MEUWISSEN T, HAYES B, GODDARD M. Genomic selection: a paradigm shift in animal breeding[J]. Animal Front., 2016, 6(1):6-14. |
11 | GAO C. Genome engineering for crop improvement and future agriculture[J]. Cell, 2021,184(6):1621-1635. |
12 | LEE K, UH K, FARRELL K. Current progress of genome editing in livestock[J]. Theriogenology, 2020,150:229-235. |
13 | ROELL M S, ZURBRIGGEN M D. The impact of synthetic biology for future agriculture and nutrition[J]. Curr. Opin. Biotechnol., 2020,61:102-109. |
14 | AVERY O, MACLEOD C, MCCARTY M. Studies on the chemical nature of the substance inducing transformation of pneumococcal types. Induction of transformation by a desoxyribonucleic acid fraction isolated from pneumococcus type Ⅲ[J]. J. Exp. Med.,1944,79(2): 137-158. |
15 | CHARGAFF E, ZAMENHOF S, GREEN C. Human desoxypentose nucleic acid: composition of human desoxypentose nucleic acid[J]. Nature,1950,165: 756-757 |
16 | WATSON J D, CRICK F H. Genetical implications of the structure of deoxyribonucleic acid[J]. Nature,1953,171(4361):964-967. |
17 | SZYMANSKI M, BARCISZEWSKI J. The path to the genetic code[J]. Biochim. Biophys. Acta Gen. Subj., 2017,1861(11):2674-2679. |
18 | COHEN S N, CHANG A C, BOYER H W, et al.. Construction of biologically functional bacterial plasmids in vitro[J]. Proc. Natl. Acad. Sci. USA,1973,70(11):3240-3244. |
19 | MORROW J F, COHEN S N, CHANG A C, et al.. Replication and transcription of eukaryotic DNA in Escherichia coli [J]. Proc. Natl. Acad. Sci. USA, 1974,71(5):1743-1747. |
20 | GORDON J W, RUDDLE F H. Integration and stable germ line transmission of genes injected into mouse pronuclei[J]. Science, 1981,214:1244-1246. |
21 | PETRI W. Transgenic organisms and development[J]. Nature,1982, 299:399-400, |
22 | PALMITER R, BRINSTER R, HAMMER R, et al.. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes[J]. Nature,1982,300:611-615. |
23 | HERNALSTEENS J P, FVAN VLIET, DE BEUCKELEER M, et al.. The Agrobacterium tumefaciens Ti plasmid as a host vector system for introducing foreign DNA in plant cells[J]. Nature, 1980,287:654-656. |
24 | OTTEN L, DE GREVE H, HERNALSTEENS J P, et al.. Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens [J]. Mol. Gen. Genet., 1981,183(2):209-213. |
25 | FRALEY R T, ROGERS S G, HORSCH R B, et al.. Expression of bacterial genes in plant cells[J]. Proc. Natl. Acad. Sci. USA, 1983,80(15):4803-4807. |
26 | LAMPPA G, NAGY F, CHUA N H. Light-regulated and organ-specific expression of a wheat Cab gene in transgenic tobacco[J]. Nature,1985,316:750-752. |
27 | International Service for the Acquisition of Agri-biotech Applications (ISAAA). Global status of commercialized biotech/GM crops in 2019[J/OL]. ISAAA,2019[2021-06-18]. . |
28 | 朱祯, 刘哲铭, 李跃. 转基因技术的前世今生[J].人与自然圈,2018,114:1-16. |
29 | 陈章良. 农业生物技术研究及产业的现状和我国发展策略的几点考虑[J]. 中国农业科技导报,1999,1(1):18-20. |
30 | 林敏. 转基因争论需要科学探索理性讨论[J]. 人与生物圈,2018(6):72-75. |
31 | 陈君石. 助也媒体,误也媒体——点评2010中国十大健康事件[J]. 健康管理,2011(1):44-45. |
32 | 万建民. 我国转基因技术研究现状[J]. 民主与科学, 2015,5:20-21. |
33 | 农业部科技教育司. 中国农业科学技术70年[M]. 北京:中国农业出版社,2019. |
34 | 熊明民,杨亚岚,阮进学,等. 我国动物生物育种产业现状及发展策略探讨[J]. 农业生物技术学报,2016(8):1199-1206. |
35 | NESS J E, CARDAYRÉ S B, MINSHULL J, et al.. Molecular breeding: the natural approach to protein design[J]. Adv. Protein Chem., 2000,55:261-292. |
36 | MCPHERSON M J, HARRISON D J. Protease inhibitors and directed evolution: enhancing plant resistance to nematodes[J]. Biochem. Soc. Symp., 2001(68):125-142. |
37 | PELEMAN J D, VAN DER V J R. Breeding by design[J]. Trends Plant Sci., 2003,8(7):330-334. |
38 | WANG Y, XUE Y, LI J. Towards molecular breeding and improvement of rice in China[J]. Trends Plant Sci., 2005,12:610-614. |
39 | VARSHNEY R K, LANGRIDGE P, GRANER A. Application of genomics to molecular breeding of wheat and barley[J]. Adv. Genet., 2007,58:121-155. |
40 | 张启发. 绿色超级稻的培育的设想[J]. 分子植物育种,2005(5):601-602. |
41 | ZHANG Q.Strategies for developing Green Super Rice[J]. Proc. Natl. Acad. Sci. USA,2007,104(42):16402-16409. |
42 | 邓兴旺,王海洋,唐晓艳,等.杂交水稻育种将迎来新时代[J].中国科学:生命科学,2013,43:864-868. |
43 | MORRIS S H. EU biotech crop regulations and environmental risk: a case of the emperor's new clothes?[J]. Trends Biotechnol., 2007,25:2-6. |
44 | KALAITZANDONAKES N, ALSTON J M, BRADFORD K J. Compliance costs for regulatory approval of new biotech crops[J]. Nat. Biotechnol.,2007,25:509-511. |
45 | MARRIS E. Biotech crop rules get rewrite[J]. Nature,2007,449:9. |
46 | CLIVE J. A global overview of biotech (GM) crops: adoption, impact and future prospects[J]. GM Crops, 2010,1:8-12. |
47 | PRIVALLE L S, CHEN J, CLAPPER G, et al.. Development of an agricultural biotechnology crop product: testing from discovery to commercialization[J]. J. Agric. Food Chem., 2012,60:10179-10187. |
48 | JOHNSON J M, FRANZLUEBBERS A J, WEYERS S L, et al.. Agricultural opportunities to mitigate greenhouse gas emissions[J]. Environ. Pollut., 2007,150(1):107-124. |
49 | HARINDINTWALI J D, ZHOU J, MUHOZA B, et al.. Integrated eco-strategies towards sustainable carbon and nitrogen cycling in agricujlture[J/OL]. J. Environ. Manage., 2021,293(26):112856[2021-06-18]. . |
50 | GHOSH A, MISRA S, BHATTACHARYYA R, et al.. Agriculture, dairy and fishery farming practices and greenhouse gas emission footprint: a strategic appraisal for mitigation[J]. Environ. Sci. Pollut. Res. Int., 2020, 27(10):10160-10184. |
51 | 李新海,谷晓峰,马有志,等.农作物基因设计育种发展现状与展望[J]. 中国农业科技导报,2020,22(8):1-4. |
52 | TIAN Z, WANG J W, LI J, et al.. Designing future crops: challenges and strategies for sustainable agriculture[J]. Plant J., 2021,105(5):1165-1178. |
53 | VOIGT C A. Synthetic biology 2020-2030: six commercially-available products that are changing our world[J/OL]. Nat. Commun., 2020, 11(1): 6379[2021-06-18]. . |
54 | MEUWISSEN T H, HAYES B, GODDARD M E. Prediction of total genetic value using genome-wide dense marker maps[J]. Genetics, 2001,157:1819-1829. |
55 | 马宇浩,高爽,董向会,等. 基因编辑在农业动物中的应用进展[J].农业生物技术学报,2020,28(12):2230-2239. |
56 | ZHU H, LI C, GAO C. Applications of CRISPR-Cas in agriculture and plant biotechnology[J]. Nat. Rev. Mol. Cell Biol., 2020,21(11):661-677. |
57 | ANZALONE A V, KOBLAN L W, LIU D R. Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors[J]. Nat. Biotechnol., 2020,38(7):824-844. |
58 | 宗媛,高彩霞.碱基编辑系统研究进展[J]. 遗传,2019,41(9): 777-800. |
59 | LI C, ZHANG R, MENG X, et al.. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors[J]. Nat. Biotechnol., 2020,38(7):875-882. |
60 | ANZALONE A V, RANDOLPH P B, DAVIS J R, et al.. Search-and-replace genome editing without double-strand breaks or donor DNA[J]. Nature, 2019,576(7785):149-157. |
61 | HUANG T K, PUCHTA H. Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering[J/OL]. Transgenic Res., 2021, doi: 10.1007/s11248-021-00238-x,[2021-06-18]. . |
62 | LIN Q, ZONG Y, XUE C, et al.. Prime genome editing in rice and wheat[J]. Nat. Biotechnol., 2020,38(5):582-585. |
63 | LIN Q, JIN S, ZONG Y, et al.. High-efficiency prime editing with optimized, paired pegRNAs in plants[J/OL]. Nat. Biotechnol., 2021, doi: 10.1038/s41587-021-00868-w[2021-06-18]. . |
64 | THAKORE P I, BLACK J B, HILTON I B, et al.. Editing the epigenome: technologies for programmable transcription and epigenetic modulation[J]. Nat. Methods, 2016,13:127-137. |
65 | NUÑEZ J K, CHEN J, POMMIER G C, et al.. Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing[J]. Cell, 2021,184(9):2503-2519. |
66 | LV J, YU K, WEI J, et al.. Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3[J]. Nat. Biotechnol., 2020,38(12):1397-1401. |
67 | WANG B, ZHU L, ZHAO B, et al.. Development of a haploid-inducer mediated genome editing system for accelerating maize breeding[J]. Mol. Plant, 2019,12(4):597-602. |
68 | LI T, YANG X, YU Y, et al.. Domestication of wild tomato is accelerated by genome editing[J]. Nat. Biotechnol., 2018,36:1160-1163. |
69 | YU H, LIN T, MENG X B, et al.. A route to de novo domestication of wild allotetraploid rice[J]. Cell,2021,184(4):1156-1170. |
70 | 张先恩. 中国合成生物学发展回顾与展望[J]. 中国科学: 生命科学, 2019,49:1543-1572. |
71 | VOIGT C A. Synthetic biology 2020-2030: six commercially-available products that are changing our world[J/OL]. Nat. Commun., 2020,11(1):6379[2021-06-18]. . |
72 | 吴杰, 赵乔. 合成生物学在现代农业中的应用与前景[J].植物生理学报,2020,56(11):2308-2316. |
73 | 朱新广,熊燕,阮梅花,等.光合作用合成生物学研究现状及未来发展策略[J].中国科学院院刊,2018,33:1239-1248. |
74 | SVON CAEMMERER, QUICK W P, FURBANK R T. The development of C₄ rice: current progress and future challenges[J]. Science, 2012,336(6089):1671-1672. |
75 | SCHULER M L, MANTEGAZZA O, WEBER A P M. Engineering C4 photosynthesis into C3 chassis in the synthetic biology age[J]. Plant J., 2016,87(1):51-65 |
76 | BATISTA-SILVA W, FONSECA-PEREIRA P D A, MARTINS A O, et al.. Engineering improved photosynthesis in the era of synthetic biology[J/OL]. Plant Commun., 2020,1(2):100032[2021-06-18]. . |
77 | MACKINDER L C M, MEYER M T, METTLER-ALTMANN T, et al.. A repeat protein links Rubisco to form the eukaryotic carbon-concentrating organelle[J]. Proc. Natl. Acad. Sci. USA, 2016,113(21):5958-5963. |
78 | LONG B M, HEE W Y, SHARWOOD R E, et al.. Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts[J/OL]. Nat. Commun., 2018,9(1):3570[2021-06-18]. . |
79 | SOUTH P F, CAVANAGH A P, LIU H W, et al.. Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field[J/OL]. Science, 2019,363(6422): eaat9077[2021-06-18]. . |
80 | 燕永亮, 王忆平, 林敏.生物固氮体系人工设计的研究进展[J].生物产业技术, 2019,1:34-40. |
81 | 林章凛,林敏.微生物和植物抗逆性元器件的合成生物学研究[J].生物产业技术,2013,4:7-17. |
82 | GOOD A. Toward nitrogen-fixing plants:a concerted research effort could yield engineered plants that can directly fix nitrogen[J]. Science,2018, 359(6378):869-870. |
83 | ROGERS C, OLDROYD G E D. Synthetic biology approaches to engineering the nitrogen symbiosis in cereals[J]. J. Exp. Bot., 2014,65(8):1939-1946. |
84 | ORTIZ-MARQUEZ J C, NASCIMENTO M D O, CURATTI L. Metabolic engineering of ammonium release for nitrogen-fixing multispecies microbial cell-factories[J]. Metab. Eng., 2014,23:154-164. |
85 | ZHANG T, YAN Y, HE S, et al.. Involvement of the ammonium transporter AmtB in nitrogenase regulation and ammonium excretion in Pseudomonas stutzeri A1501[J]. Res. Microbiol., 2012,163:332-339. |
86 | ZHANG S, HEYES D J, FENG L L, et al.. Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis[J]. Nature, 2019,574(7780):722-725. |
87 | ZHAN Y H, YAN Y L, et al.. The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501[J]. Proc. Natl. Acad. Sci. USA, 2016,113(30):4348-4356. |
88 | YANG J G, XIE X Q, YANG M X, et al.. Modular electron-transport chains from eukaryotic organelles function to support nitrogenase activity[J]. Proc. Natl. Acad. Sci. USA, 2017,114(12):E2460-E2465. |
89 | YANG J G, XIE X Q, XIANG N, et al.. Polyprotein strategy for stoichiometric assembly of nitrogen fixation components for synthetic biology[J]. Proc. Natl. Acad. Sci. USA,2018,115(36):E8509-E8517. |
90 | SEXTON A E, GARNETT T, LORIMER J. Framing the future of food: The contested promises of alternative proteins[J/OL]. Environ. Plan. E Nat. Space, 2019,doi:10.1177/2514848619827009[2021-06-18]. . |
91 | STEPHENS N, DI SILVIO L, DUNSFORD I, et al.. Bringing cultured meat to market: technical, socio-political, and regulatory challenges in cellular agriculture[J]. Trends Food Sci. Technol., 2018,78:155-166. |
92 | BJÖRN W, PRZEMEK O, SEDEF K, et al.. Food for thought: the protein transformation[R/OL]. Boston Consulting Group, [2021-03-24]. . |
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