基因编辑产品检测技术研究进展

doi:10.19586/j.2095-2341.2021.0037

生物技术进展 ›› 2021, Vol. 11 ›› Issue (4): 438-445.DOI: 10.19586/j.2095-2341.2021.0037

基因编辑产品检测技术研究进展

王梦雨¹(), 王颢潜², 王旭静¹, 王志兴¹()

^1.中国农业科学院生物技术研究所，农业农村部农业转基因生物安全评价（分子）重点实验室，北京 100081
^2.农业农村部科技发展中心，北京 100176

收稿日期:2021-03-23 接受日期:2021-04-22 出版日期:2021-07-25 发布日期:2021-08-02
通讯作者: 王志兴
作者简介:王梦雨 E-mail：wangmengyu@cau.edu.cn；

Research Progress of Gene Editing Products Detection Technology

Mengyu WANG¹(), Haoqian WANG², Xujing WANG¹, Zhixing WANG¹()

^1.Key Laboratory of Genetically Modified Organisms Safety Evaluation （Molecule），Ministry of Agriculture and Rural Affairs，Biotechnology Research Institute，Chinese Academy of Agricultural Sciences，Beijing 100081，China
^2.Development Center for Science and Technology，Ministry of Agriculture and Rural Affairs，Beijing 100176，China

Received:2021-03-23 Accepted:2021-04-22 Online:2021-07-25 Published:2021-08-02
Contact: Zhixing WANG

摘要/Abstract

摘要：

当前国内外生物技术产业革命加速推进，以基因编辑为代表的转基因新技术发展迅猛，新基因、新性状、新产品不断涌现。围绕基因编辑产品的检测方法将成为转基因生物安全监管的重要一环和有效支撑。因此，基于测序技术、酶切技术、PCR技术和其他检测技术4个方面，总结了目前应用于基因编辑产品检测的一些技术方法，并对每种方法的优缺点进行了分析，以期为今后基因编辑产品的检测提供思路，做好转基因监管技术储备。

关键词: 基因编辑, 检测技术, 下一代测序, TaqMan方法

Abstract:

At present， with the acceleration of the biotechnology industry revolution at home and abroad， the new transgenic technologies represented by gene editing are developing rapidly， and new genes， new traits and new products are constantly emerging. The detection method of gene editing products will become an important link and effective support for the safety supervision of genetically modified organisms. Therefore， based on sequencing， enzyme digestion， PCR and other detection technologies， some technical methods applied to the detection of gene editing products at present were summarized， and the advantages and disadvantages of each method were analyzed， in order to provide ideas for the detection of gene editing products in the future and make a good reserve of genetically modified regulatory technologies.

Key words: gene editing, detection technology, NGS, TaqMan

中图分类号:

王梦雨, 王颢潜, 王旭静, 王志兴. 基因编辑产品检测技术研究进展[J]. 生物技术进展, 2021, 11(4): 438-445.

Mengyu WANG, Haoqian WANG, Xujing WANG, Zhixing WANG. Research Progress of Gene Editing Products Detection Technology[J]. Current Biotechnology, 2021, 11(4): 438-445.

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基因编辑产品检测技术研究进展

Research Progress of Gene Editing Products Detection Technology

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