数字PCR在食源性致病菌检测中的应用进展

doi:10.19586/j.2095-2341.2021.0001

生物技术进展 ›› 2021, Vol. 11 ›› Issue (3): 260-268.DOI: 10.19586/j.2095-2341.2021.0001

数字PCR在食源性致病菌检测中的应用进展

卢海强1,焦新雅1,2,吴思源2,王娅丽2,刘璐2,程书梅1,张晓3*,苏晓峰2*

1.河北农业大学食品科技学院，河北保定 071001;

2.中国农业科学院生物技术研究所，北京 100081;

3.长春理工大学生命科学技术学院，长春 130022

收稿日期:2021-01-04 出版日期:2021-05-25 发布日期:2021-02-07
通讯作者: 张晓 E-mail: zhangxiao@cust.edu.cn；苏晓峰 E-mail: suxiaofeng@caas.cn
作者简介:卢海强 E-mail：luhaiqiang@hebau.edu.cn
基金资助:
国家自然科学基金青年科技基金项目（31901631）。

Application Progress on the Digital PCR in Detection of Foodborne Pathogenic Bacteria

LU Haiqiang, JIAO Xinya^,, WU Siyuan, WANG Yali, LIU Lu, CHENG Shumei, ZHANG Xiao, SU Xiaofeng

1.College of Food Science and Technology, Hebei Agricultural University, Hebei Baoding 071001, China;

2.Biotechnology Research Institute, Chinese Academy of Agricultural Science, Beijing 100081, China;

3.School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China

Received:2021-01-04 Online:2021-05-25 Published:2021-02-07

摘要/Abstract

摘要： 食源性致病菌是食品安全的重大隐患，对人类健康造成极大危害，因此亟待研究和建立精准有效的食源性致病菌检测方法。随着单分子检测技术的快速发展，数字PCR技术因其具有超高的灵敏度、稳定性和低试剂消耗等优点而被广泛应用于食源性致病菌的检测。主要介绍了数字PCR的基本原理及其研究进展，深入探讨了其在检测大肠杆菌（Escherichia coli）、沙门氏菌（Salmonella）、金黄色葡萄球菌（Staphylococcus aureus）、空肠弯曲菌（Campylobacter jejuni）、志贺氏菌（Shigella）、克罗诺杆菌（Cronobacter）、副溶血性弧菌（Vibrio parahaemolyticus）、单核细胞增生李斯特氏菌（Listeria monocytogenes）和蜡状芽孢杆菌（Bacillus cereus）中的应用，为今后该技术在食源性致病菌检测中的研究与应用提供一定的技术性参考。

关键词: 食源性致病菌检测, 数字PCR, 微滴数字PCR

Abstract: Foodborne pathogenic bacteria are the major risk of food safety, causing great harm to human health. Thus, it is urgent to research and establish an accurate and effective detection method for foodborne pathogenic bacteria. With the rapid development of single-molecule techniques, digital PCR has been widely used in detection of foodborne pathogenic bacteria due to their outstanding advantages, such as high sensitivity, high stability and low reagent consumption. This review mainly summarized the basic principles and research progress of digital PCR. The applications of digital PCR technology in detection of Escherichia coli, Salmonella, Staphylococcus aureus, Campylobacter jejuni, Shigella, Cronobacter, Vibrio parahaemolyticus, Listeria monocytogenes and Bacillus cereus were also discussed, which would provide further insight into the detection of foodborne pathogenic bacteria.

Key words: detection of foodborne pathogenic bacteria, digital PCR, droplet digital PCR

卢海强,焦新雅,,吴思源,王娅丽,刘璐,程书梅,张晓,苏晓峰. 数字PCR在食源性致病菌检测中的应用进展[J]. 生物技术进展, 2021, 11(3): 260-268.

LU Haiqiang1, JIAO Xinya1,2, WU Siyuan2, WANG Yali2, LIU Lu2, CHENG Shumei1, ZHANG Xiao3*, SU Xiaofeng2*. Application Progress on the Digital PCR in Detection of Foodborne Pathogenic Bacteria[J]. Curr. Biotech., 2021, 11(3): 260-268.

[1]	门佩璇, 肖宇锋, 张玢. 基于计量学方法分析数字PCR技术的临床应用现状与技术热点[J]. 生物技术进展, 2022, 12(4): 606-613.
[2]	郑子繁,柳方方,刘卫晓,金芜军,李亮. 数字PCR技术在核酸标准物质研制中的应用[J]. 生物技术进展, 2020, 10(6): 579-584.
[3]	王尚君,董莲华. 数字PCR仪校准方法研究[J]. 生物技术进展, 2020, 10(6): 585-589.
[4]	胡思宏,游国叶. 数字PCR在新型冠状病毒检测中的应用前景[J]. 生物技术进展, 2020, 10(6): 674-679.
[5]	纪艺,陈笑芸,丁霖,汪小福,彭城,徐晓丽,徐俊锋. 肉类及肉制品中动物源性成分鉴别方法研究进展[J]. 生物技术进展, 2020, 10(6): 711-716.
[6]	刘晓,朱鹏宇,景小艳,李志红,张永江,李想,付伟,. 双重数字PCR在转基因大豆检测中的应用[J]. 生物技术进展, 2020, 10(1): 60-66.

数字PCR在食源性致病菌检测中的应用进展

Application Progress on the Digital PCR in Detection of Foodborne Pathogenic Bacteria

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价