关键词: 数字PCR, 校准

Abstract: The digital PCR instrument is an important instrument for absolute quantification of nucleic acids, and it is of great significance to ensure the accuracy of the detection results of the digital PCR instrument. Different digital PCR instruments on the domestic market were compared, the performance indexes of the digital PCR instrument were analyzed and the calibration method of the instrument were discussed. Several key measurement technical indicators of the digital PCR machine calibration were set, including the copy number concentration relative indication error, copy number concentration repeatability, fluorescence channel consistency and the number of reaction unit repeatability. The method was tested and verified by utilizing traceable national certified reference materials. The verification results showed the feasibility of the calibration method.  The method has strong operability, can meet the technical requirements of the instrument and the needs of users, improve the accuracy and reliability of the detection results of the digital PCR instrument, and has a positive role in further expanding and deepening the application of the digital PCR technology.

Key words: digital PCR, calibration