基因编辑技术在植物启动子编辑中的研究进展

doi:10.19586/j.2095-2341.2023.0001

生物技术进展 ›› 2023, Vol. 13 ›› Issue (3): 321-328.DOI: 10.19586/j.2095-2341.2023.0001

• 进展评述 • 下一篇

基因编辑技术在植物启动子编辑中的研究进展

盖思宇¹(), 陈子奇²(), 夏涵超¹, 赵仁贵¹(), 刘相国²()

^1.吉林农业大学农学院，长春 130118
^2.吉林省农业科学院，长春 130119

收稿日期:2023-01-06 接受日期:2023-02-22 出版日期:2023-05-25 发布日期:2023-06-12
通讯作者: 赵仁贵,刘相国
作者简介:盖思宇E-mail：1459365568@qq.com
陈子奇E-mail：549177102@qq.com；第一联系人： 盖思宇和陈子奇为本文共同第一作者。
基金资助:
吉林省自然科学基金项目(20220101330JC);吉林省预算内基本建设资金（创新能力建设）项目(2022C037-3);长春市现代农业发展科技支撑计划项目(21ZY13)

Research Progress of CRISPR/Cas9 Technology in Plant Promoter Editing

Siyu GAI¹(), Ziqi CHEN²(), Hanchao XIA¹, Rengui ZHAO¹(), Xiangguo LIU²()

^1.Faculty of Agronomy，Jilin Agricultural University，Changchun 130118，China
^2.Jilin Academy of Agricultural Sciences，Changchun 130119，China

Received:2023-01-06 Accepted:2023-02-22 Online:2023-05-25 Published:2023-06-12
Contact: Rengui ZHAO,Xiangguo LIU

摘要/Abstract

摘要：

植物基因的表达决定了植物的表型特征，而基因的表达受启动子的直接调控。启动子作为基因的一个组成部分，控制着基因表达(转录)的起始时间和表达程度。利用基因编辑技术对启动子进行定向编辑之后，会因为基因序列特有的重组排列、顺式表达等因素使得植物中的某个或某些基因的表达模式发生改变，进而影响基因功能。这些改变最终直接或间接地改变了植物的外在表型特征，而一些正向改变会对植物的品质起到优化和改良作用。综合近几年基因编辑技术对启动子的研究，主要从启动子的构成与分类、基因编辑技术和启动子编辑的研究进展这3个方面对启动子的编辑在植物中的应用进行了概述和总结，以期为启动子编辑技术应用于植物改良提供参考。

关键词: 基因编辑技术, 启动子编辑, 植物品质改良

Abstract:

Plant gene expression determines plant phenotypic characteristics， and gene expression is directly regulated by promoters. As a component of genes， promoters control the initiation time and expression degree of gene expression （transcription）. After directed editing of promoters by gene editing technology， the expression pattern of one or some genes in plants would be changed due to the unique recombination and arrangement of gene sequences， cis-expression and other factors， thus affecting the function of genes. These changes ultimately directly or indirectly change the external phenotypic characteristics of plants， and positive changes would help to optimize and improve plant quality. In this paper， the application of promoter editing in plants was summarized from three aspects： the structure and classification of promoters， gene editing technology and the research progress of promoter editing， which was expected to provide reference for plant improving by promoter editing.

Key words: gene editing technology, promoter editing, plant quality improvement

中图分类号:

盖思宇, 陈子奇, 夏涵超, 赵仁贵, 刘相国. 基因编辑技术在植物启动子编辑中的研究进展[J]. 生物技术进展, 2023, 13(3): 321-328.

Siyu GAI, Ziqi CHEN, Hanchao XIA, Rengui ZHAO, Xiangguo LIU. Research Progress of CRISPR/Cas9 Technology in Plant Promoter Editing[J]. Current Biotechnology, 2023, 13(3): 321-328.

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基因编辑技术在植物启动子编辑中的研究进展

Research Progress of CRISPR/Cas9 Technology in Plant Promoter Editing

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