矿质元素和植物激素对超群羊肚菌生长影响的研究

doi:10.19586/j.2095-2341.2024.0097

生物技术进展 ›› 2024, Vol. 14 ›› Issue (6): 947-951.DOI: 10.19586/j.2095-2341.2024.0097

• 食药用菌生物技术专题 • 上一篇下一篇

矿质元素和植物激素对超群羊肚菌生长影响的研究

章寅¹(), 李志明², 晁盛茜¹, 陈一帆¹, 吕贝贝¹()

^1.上海市农业科学院生物技术研究所，农业农村部农作物生态环境安全检验测试中心（上海），上海市农业生物安全评价与检测专业技术服务平台，农业农村部农业转基因生物安全评价（环境）重点实验室，上海市农业遗传育种重点实验室，上海 201106
^2.台州学院生命科学学院，浙江台州 318000

收稿日期:2024-05-07 接受日期:2024-07-24 出版日期:2024-11-25 发布日期:2024-12-27
通讯作者: 吕贝贝
作者简介:章寅 E-mail: zhangyinsy1986@126.com；
基金资助:
国家自然科学基金项目(32302635);上海市科委“一带一路”国际合作项目(20310750500);上海市自然科学基金(22ZR1442500);上海市农业科学院农业科技创新支撑领域专项〔农科应基2024（08）〕;上海市农业科学院卓越团队〔沪农科卓（2022）016〕

Effect of Mineral Elements and Phytohormones on the Growth of Morchella exima

Yin ZHANG¹(), Zhiming LI², Shengqian CHAO¹, Yifan CHEN¹, Beibei LYU¹()

^1.Key Laboratory of Safety Evaluation of Agricultural Genetically Modified Organisms （Environment），Ministry of Agriculture and Rural Affairs，Shanghai Key Laboratory of Agricultural Genetics and Breeding，Test Center for Ecological Environment Safety of Crops of MOA（Shanghai），Shanghai Professional Technology Service Platform of Agricultural Biosafety Evaluation and Testing，Biotech Research Institute，Shanghai Academy of Agricultural Sciences，Shanghai 201106，China
^2.College of Life Sciences，Taizhou University，Zhejiang Taizhou 318000，China

Received:2024-05-07 Accepted:2024-07-24 Online:2024-11-25 Published:2024-12-27
Contact: Beibei LYU

摘要/Abstract

摘要：

研究旨在探讨矿质元素和植物激素对超群羊肚菌菌丝生长和多糖产生的影响。以超群羊肚菌菌株为测试材料，以该菌株在不同质量浓度培养基上生长的菌落直径为考察指标，探究矿质元素和植物激素质量浓度对羊肚菌菌丝生长的影响；再从上述浓度梯度中选取菌丝生长较优的质量浓度，配置成培养基对羊肚菌进行培养，测定其多糖含量。结果显示，CaSO₄在一定的浓度范围内对羊肚菌菌丝的生长起到促进作用，在质量浓度为10 g·L^-1时促进作用最为明显；低浓度MgSO₄（<0.1 g·L^-1）对羊肚菌的菌丝生长起到促进作用，质量浓度为0.01 g·L^-1时促进作用最明显，浓度过高则出现抑制且随质量浓度升高抑制作用越明显；植物激素水杨酸对羊肚菌的生长无明显作用；适量质量浓度（0.005～0.05 mg·L^-1）的茉莉酸甲酯能促进羊肚菌菌丝的生长。一定质量浓度的CaSO₄（10 g·L^-1左右）可以促使羊肚菌产生胞外多糖；MgSO₄质量浓度越高，羊肚菌胞外多糖的总量越高。适量质量浓度的CaSO₄、MgSO₄和茉莉酸甲酯都能促进羊肚菌菌丝的生长，水杨酸对羊肚菌的生长无影响。CaSO₄对羊肚菌产生胞外多糖表现为双重作用，既可以抑制羊肚菌中胞外多糖产生也可促进其产生；MgSO₄对胞外多糖的产生则表现为促进作用。

关键词: 羊肚菌, 矿质元素, 植物激素, 多糖

Abstract:

The effects of mineral elements and phytohormones on mycelial growth and extracellular polysaccharide production of Morchella were discussed. The Morchella spp. was used as the test material. The diameter of colony on the different media was used as the inspection index to study the effect of different concentrations of mineral elements and hormones on mycelial growth of Morchella mycelium. Then， from each substance， several preferred mass concentrations and configure the culture medium were selected according to these mass concentrations to culture Morchella esculenta， and measured the polysaccharide content was measured. Results showed that， CaSO₄ in mineral elements played an important role in promoting the growth of Morchella esculenta. The effect was most obvious when the mass concentration was 10 g·L^-1. Low concentrations of MgSO₄ （<0.1 g·L^-1） promoted the growth of Morchella. At the concentration of 0.01 g·L^-1， the promotion effect was the strongest occured， and when the concentration was too high， the inhibition effect appeared and the inhibitory effect becomed more and more obvious with increasing concentration； salicylic acid had no obvious effect on the growth of Morchella. Methyl jasmonate at a suitable mass concentration （0.005~0.050 mg·L^-1） could promote the growth of Morchella mycelia. A certain concentration of CaSO₄ （about 10 g·L^-1） could promote the production of extracellular polysaccharides from Morchella. The higher the mass concentration of MgSO₄ was， the higher the total amount of extracellular polysaccharides of Morchella was. Appropriate concentration of CaSO₄， MgSO₄ and methyl jasmonate can promote the growth of Morchella mycelium， and salicylic acid had no effect on the growth of Morchella. CaSO₄ has a dual effect on the production of extracellular polysaccharides from Morchella. it not only inhibits the production of extracellular polysaccharides， but also promotes the production of extracellular polysaccharides. MgSO₄ shows a promotion effect on the production of extracelluar poly saccharicles.

Key words: Morchella exima, mineral elements, hormones, polysaccharides

中图分类号:

Q949.3

章寅, 李志明, 晁盛茜, 陈一帆, 吕贝贝. 矿质元素和植物激素对超群羊肚菌生长影响的研究[J]. 生物技术进展, 2024, 14(6): 947-951.

Yin ZHANG, Zhiming LI, Shengqian CHAO, Yifan CHEN, Beibei LYU. Effect of Mineral Elements and Phytohormones on the Growth of Morchella exima[J]. Current Biotechnology, 2024, 14(6): 947-951.

图/表 6

图 1 CaSO4对羊肚菌菌丝生长的影响注：不同小写字母表示处理间有统计学差异（P<0.05）。

Fig. 1 Effect of CaSO4 on the growth of Morchella

图 2 MgSO4对羊肚菌菌丝生长的影响注：不同小写字母表示处理间有统计学差异（P<0.05）。

Fig. 2 Effect of MgSO4 on the growth of Morchella

图 3 水杨酸对羊肚菌菌丝生长的影响注：不同小写字母表示处理间有统计学差异（P<0.05）。

Fig. 3 Effect of salicylic acid on the growth of Morchella

图 4 茉莉酸甲酯对羊肚菌菌丝生长的影响注：不同小写字母表示处理间有统计学差异（P<0.05）。

Fig. 4 Effect of methyl jasmonate on the growth of Morchella

图 5 CaSO4对羊肚菌产生多糖总量的影响注：不同小写字母表示处理间有统计学差异（P<0.05）。

Fig. 5 Effect of CaSO4 on total polysaccharide produced by Morchella

图 6 MgSO4对羊肚菌产生多糖总量的影响注：不同小写字母表示处理间有统计学差异（P<0.05）。

Fig. 6 Effect of MgSO4 on total polysaccharide produced by Morchella

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矿质元素和植物激素对超群羊肚菌生长影响的研究

Effect of Mineral Elements and Phytohormones on the Growth of Morchella exima

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