关键词: 南五味子多糖, 响应面分析法, 超声辅助酶法提取

Abstract: In this study, the ultrasonic-assisted enzymatic extraction was employed to extract polysaccharides from Kadsura longipedunculata and the extraction process was also studied. Based on single factor experiments, the response surface methodology (RSM) was carried out to further optimize extraction conditions. The optimal extraction condition were extraction temperature 57℃, extraction time 66 min, extraction pH 4.8, enzyme dosage 3.0%. Under the optimum conditions, the yield of crude polysaccharide was 8.73%±0.25%. In summary, the extraction process involved in the study could significantly reduce polysaccharide extraction time and solvent consumption, reduce temperature and effectively improve the extraction yield. Therefore, this process can be applied in the extraction of polysaccharides from Kadsura longipedunculata. The results provided a scientific basis for developing and utilization of polysaccharide from Kadsura longipedunculata.

Key words: polysaccharide from Kadsura longipedunculata, response surface methodology, ultrasonic assisted enzymatic extraction