关键词: 治疗性糖蛋白药物, 多糖分析, 凝集素芯片技术

Abstract: Therapeutic monoclonal antibodies (mAbs) are glycoproteins produced by living cell systems. The glycan moieties attached to the proteins can directly affect protein stability, bioactivity and immunogenicity. Therefore, glycan variants of a glycoprotein product must be adequately analyzed and controlled to ensure product quality. However, the inherent complexity of protein glycosylation poses a daunting analytical challenge. This review provided an update of recent advances in glycan analysis, including the potential utility of lectin-based microarray for high throughput glycan proling. Emphasis was placed on comparison of the major types of analytics for use in determining unique glycan features such as glycosylation site, glycan structure and content, which was expected to provide reference for research of glycoproteins.

Key words: therapeutic glycoproteins, glycan analysis, lectin microarray