关键词: 红树林真菌, 淡紫拟青霉, 胞外多糖

Abstract: To explore the optimum extraction conditions of extracellular polysaccharides from Paecilomyces lilacinuson. Several key factors that affect harvest of the compound, such as concentration times of fermentation supernatant, concentration of trichloroacetic acid, and so on, were determined as single factor experiment. L9(34) orthogonal assay based on factors of ethanol concentration, extraction temperature and time was designed. Results showed that the optimum parameters were as follows: concentrated material-the original material ratio 3~5∶1, 95% ethanol-material ratio 3∶1, precipitation time and temperature: 24 h, 4 ℃ respectively. In this condition, extraction ratio of extracellular polysaccharides could reach 57.835%±1.206%. The results provided reference for extraction of extracellular polysaccharides from mangrove fungus Paecilomyces lilacinuson.

Key words: mangrove fungus, Paecilomyces lilacinuson, extracellular polysaccharide