香菇多糖结构与功能研究进展

doi:10.19586/j.2095-2341.2024.0127

生物技术进展 ›› 2024, Vol. 14 ›› Issue (6): 911-919.DOI: 10.19586/j.2095-2341.2024.0127

• 食药用菌生物技术专题 • 上一篇下一篇

香菇多糖结构与功能研究进展

董浩然(), 姜宁, 陆欢, 付阳, 李巧珍(), 于海龙()

上海市农业科学院食用菌研究所，国家食用菌工程技术研究中心，农业农村部南方食用菌资源利用重点实验室，上海 201403

收稿日期:2024-07-12 接受日期:2024-09-27 出版日期:2024-11-25 发布日期:2024-12-27
通讯作者: 李巧珍,于海龙
作者简介:董浩然 E-mail： dhr605061238@163.com
基金资助:
国家现代农业产业技术体系项目(CARS-20);山东省重点研发项目(20221ZGC023);上海市农业科学院卓越团队〔沪农科卓(2022)001〕;山东省泰山产业领军人才项目(LJNY202116)

Research Progress on Structure and Function of Lentinan

Haoran DONG(), Ning JIANG, Huan LU, Yang FU, Qiaozhen LI(), Hailong YU()

Key Laboratory of Edible Fungi Resources and Utilization（South），Ministry of Agriculture and Rural Affairs，National Engineering Research Center of Edible Fungi，Institute of Edible Fungi，Shanghai Academy of Agricultural Sciences，Shanghai 201403，China

Received:2024-07-12 Accepted:2024-09-27 Online:2024-11-25 Published:2024-12-27
Contact: Qiaozhen LI,Hailong YU

摘要/Abstract

摘要：

香菇（Lentinula edodes）作为我国重要食用菌，具有悠久的栽培历史，如今在全球广泛应用于食品和药用领域。香菇富含生物活性多糖，其中β-葡聚糖的含量尤为丰富。自20世纪70年代以来，人们发现香菇多糖的抗癌生物活性之后，针对香菇多糖的活性研究从未停止。研究表明，香菇多糖具有多种显著的生物活性，涵盖抗氧化、抗肿瘤、抗衰老、抗炎、免疫调节、抗病毒、保肝以及降胆固醇等功效。香菇多糖的分离提纯方法、化学性质与生物活性一直备受世界各地学者的密切关注。基于前人的工作基础，全面总结了香菇多糖的最新研究进展，包括香菇多糖的分离方法及不同分离方法对其结构活性的影响、结构特征与多糖生物活性的关系以及香菇多糖在发挥生物活性时的作用机制等内容，期望为香菇多糖在医疗和功能性食品领域的进一步应用提供有价值的信息参考。

关键词: 香菇, 香菇多糖, 分离提纯, 结构特征, 生物活性

Abstract:

Lentinula edodes， as an important edible fungus in China， has a long history of cultivation. It is now widely used in the fields of food and medicine worldwide. Lentinula edodes is rich in bioactive polysaccharides， especially β-glucan. Since the discovery of the anticancer biological activity of lentinan in the 1970 s， the research on the activity of lentinan has never stopped. Studies have shown that lentinan possesses a variety of significant biological activities， including antioxidant， anti-tumor， anti-aging， anti-inflammatory， immunomodulatory， antiviral， hepatoprotective， and cholesterol-lowering effects. The isolation， purification， chemical properties， and biological activities of lentinan have always attracted close attention from scholars around the world. Based on the previous work， this article comprehensively summarized the latest research progress of lentinan， including the separation methods of lentinan and the impact of different separation methods on its structural activity， the relationship between structural characteristics and polysaccharide biological activity， and the mechanism of action of lentinan in exerting biological activity. The paper was expected to provide valuable information references for the further application of lentinan in the fields of medical treatment and functional foods.

Key words: Lentinula edodes, lentinan, separation methods, structural characteristics, bioactivity

中图分类号:

Q539

董浩然, 姜宁, 陆欢, 付阳, 李巧珍, 于海龙. 香菇多糖结构与功能研究进展[J]. 生物技术进展, 2024, 14(6): 911-919.

Haoran DONG, Ning JIANG, Huan LU, Yang FU, Qiaozhen LI, Hailong YU. Research Progress on Structure and Function of Lentinan[J]. Current Biotechnology, 2024, 14(6): 911-919.

图/表 2

参考文献 70

1	ROYSE D J, BAARS J, TAN Q. Current overview of mushroom production in the world[M]//Edible and Medicinal Mushrooms: Technology and Applications, Wiley-Blackwell, 2017, 5-13.
2	曹斌, 张月吟, 高博. 全球香菇产业发展历史、现状及趋势[J]. 食用菌学报, 2024, 31(03): 1-20.
	CAO B, ZHANG Y Y, GAO B. Development history, current situation and trends of global Lentinula edodes industry[J]. Acta Edulis Fungi, 2024, 31(03): 1-20.
3	叶雷, 清源, 吴建, 等. 不同比例桑枝屑基质栽培香菇的营养成分比较[J/OL]. 中国食物与营养, doi:10.19870/j.cnki.‍11-3716/ts.‍20231011.001 [2024-09-28]. .
	YE L, QING Y, WU J, et al.. Comparison of nutritional constituents of Lentinus edodes cultivated in different mulberry sawdust substrate[J/OL]. Food Nutr. China, doi:10.19870/j.cnki.‍11-3716/ts.‍20231011.001 [2024-09-28]. .
4	马传贵, 张志秀, 肖宝. 食用菌多糖抗肿瘤和免疫调节活性及其作用机制研究进展[C]//中国食用菌协会, 中国食用菌协会药用真菌委员会. 第十二届药用真菌学术研讨会论文集. 2023.
5	CHOWDHURY M M H, KUBRA K, AHMED S R. Screening of antimicrobial, antioxidant properties and bioactive compounds of some edible mushrooms cultivated in Bangladesh[J/OL]. Ann. Clin. Microbiol. Antimicrob., 2015, 14(1): 8[2024-10-10]..
6	LIN Y Y, ZENG H Y, WANG K, et al.. Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: process optimization,structure characterization and antioxidant activity[J]. Int. J. Biol. Macromol., 2019, 136: 305-315.
7	ELHUSSEINY S M, EL-MAHDY T S, AWAD M F, et al.. Proteome analysis and in vitro antiviral,anticancer and antioxidant capacities of the aqueous extracts of Lentinula edodes and Pleurotus ostreatus edible mushrooms[J/OL]. Molecule (basel switz.), 2021, 26(15): 4623[2024-10-15]. .
8	GARCIA J, AFONSO A, FERNANDES C, et al.. Comparative antioxidant and antimicrobial properties of Lentinula edodes Donko and Koshin varieties against priority multidrug-resistant pathogens[J]. South African J. Chem. Engin., 2021, 35(1): 98-106.
9	PATTANAYAK M, MAITY P, SAMANTA S, et al.. Studies on structure and antioxidant properties of a heteroglycan isolated from wild edible mushroom Lentinus sajor-caju[J]. Int. J. Biol. Macromol., 2018, 107: 322-331.
10	ZHANG Y, LI Q, SHU Y M, et al.. Induction of apoptosis in S180 tumour bearing mice by polysaccharide from Lentinus edodes via mitochondria apoptotic pathway[J]. J. Funct. Foods, 2015, 15: 151-159.
11	周欣. 香菇多糖提取工艺的优化及其体外抗菌试验研究[J]. 饲料研究, 2023,46(13): 87-92.
	ZHOU X. Optimization of extraction process for shiitake mushroom polysaccharides and its in vitro antibacterial test[J]. Feed. Res., 2023, 46(13): 87-92.
12	李明櫆, 王梦娜, 李占峰, 等. 基于加热回流法的香菇多糖提取工艺优化及其产品研发[J]. 食品科技, 2024, 49 (3): 210-216.
	LI M K, WANG M N, LI Z X, et al.. Optimization of Lentinan extraction process based on heating reflux method and its product development[J]. Food Sci. Technol., 2024, 49(3): 210-216.
13	赵茹, 康明丽, 张琴, 等. 酶解辅助提取香菇多糖工艺优化及其抗氧化活性[J]. 食品研究与开发, 2023, 44(12): 116-121.
	ZHAO R, KANG M L, ZHANG Q, et al.. Enzymatic hydrolvsis-assisted extraction and antioxidant activity of polvsaccharide from Lentinus edodes [J]. Food Res. Dev., 2023, 44(12): 116-121.
14	程俊文, 吴学谦, 贺亮, 等. 香菇子实体多糖分步酶解法提取研究[J]. 食用菌学报, 2009, 16(2): 67-71.
	CHENG J W, WU X Q, HE L, et al.. Extraction of polysaccharide from Lentinula edodes fruit bodies using stepwise enzymic hydrolysis[J]. Acta Edulis Fungi, 2009, 16(2): 67-71.
15	聂小宝, 张长峰, 侯成杰. 微波法辅助提取香菇多糖的工艺研究[J]. 食品工业, 2012, 33 (9): 37-39.
	NIE X B, ZHANG C F, HOU C J. Technical study on the extraction oflentinanwith microwave[J]. Food Ind., 2012, 33 (9): 37-39.
16	秦令祥, 周婧琦, 崔胜文, 等. 超声波协同复合酶法提取香菇多糖的工艺优化[J]. 食品研究与开发, 2018, 39(19): 63-67.
	QIN L X, ZHOU J Q, CUI S W, et al.. Optimization of ultrasonic-assisted compound enzyme extraction technology of lentinan[J]. Food Res. Dev., 2018, 39(19): 63-67.
17	KE L, CHEN H. Homogenate extraction of crude polysaccharides from Lentinus edodes and evaluation of the antioxidant activity[J]. Food Sci. Technol., 2016, 36: 533-539.
18	ZHANG J, WEN C, QIN W, et al.. Ultrasonic-enhanced subcritical water extraction of polysaccharides by two steps and its characterization from Lentinus edodes [J]. Int. J. Biol. Macromol., 2018, 118: 2269-2277.
19	贾薇, 余燕贞, 刘红丽, 等. 香菇子实体中高β-葡聚糖含量多糖提取物制备及体外免疫活性[J]. 食用菌学报, 2023, 30 (4): 51-58.
	JIA W, YU Y Z, LIU H L, et al.. Preparation and in vitro activity of B-Glucan-Rich polysaccharide extracts from Lentinula edodes fruiting bodies[J]. Acta Edulis Fungi, 2023, 30(4): 51-58.
20	AKRAM K, SHAHBAZ H M, KIM G R, et al.. Improved extraction and quality characterization of water-soluble polysaccharide from gamma-irradiated Lentinus edodes [J]. J. Food Sci., 2017, 82(2): 296-303.
21	李波, 芦菲, 南海娟. 香菇碱溶性多糖提取工艺的研究[J].河南科技学院学报(自然科学版), 2010, 38(2): 92-95.
	LI B, LU F, NAN H J. Study on the extraction of alkaline soluble polysaccharide in Lentinus edodes [J]. J. Henan Inst. Sci. Technol. (Nat. Sci. Ed.), 2010, 38(2): 92-95.
22	郝强, 刘晓宇, 冯睿, 等. 香菇多糖的提取数学模型研究[J].食品科技, 2007, 32(11): 87-89, 109.
	HAO Q, LIU X Y, FENG R, et al.. Study on the mathematical model of optimum yield of Lentinan polysaccharid [J]. Food Sci. Technol., 2007, 32(11): 87-89, 109.
23	YIN C, FAN X, FAN Z, et al.. Optimization of enzymes-microwave-ultrasound assisted extraction of Lentinus edodes polysaccharides and determination of its antioxidant activity[J]. Int. J. Biol. Macromol., 2018, 111: 446-454.
24	KE L Q. Optimization of ultrasonic extraction of polysaccharides from Lentinus edodes based on enzymatic treatment: Extraction of Lentinus edodes polysaccharides[J]. J. Food Proc. Preserv., 2015, 39(3): 254-259.
25	ZHANG J X, WEN C T, GU J Y, et al.. Effects of subcritical water extraction microenvironment on the structure and biological activities of polysaccharides from Lentinus edodes [J]. Int. J. Biol. Macromol., 2019, 123: 1002-1011.
26	ZHAO Y M, WANG J, WU Z G, et al.. Extraction,purification and anti-proliferative activities of polysaccharides from Lentinus edodes [J]. Int. J. Biol. Macromol., 2016, 93: 136-144.
27	TANG W, LIU C C, LIU J J, et al.. Purification of polysaccharide from Lentinus edodes water extract by membrane separation and its chemical composition and structure characterization[J/OL]. Food Hydrocoll., 2020, 105: 105851[2024-10-10]. .
28	SHI L. Bioactivities, isolation and purification methods of polysaccharides from natural products: a review[J]. Int. J. biol. macromol., 2016, 92: 37-48.
29	CHEN S P, LIU C C, HUANG X J, et al.. Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts[J]. J. Funct. Foods, 2020, 66: 103791.
30	ZHANG Q, CONG R, HU M, et al.. Immunoenhancement of edible fungal polysaccharides (lentinan,tremellan,and pachymaran) on cyclophosphamide-induced immunosuppression in mouse model[J/OL]. Evid. Based Compl. Altern. Med., 2017, 2017: 9459156[2024-10-10]. .
31	SASAKI T, TAKASUKA N. Further study of the structure of lentinan, an anti-tumor polysaccharide from Lentinus edodes [J]. Carbohydr. Res., 1976, 47(1): 99-104.
32	ZHANG L N, LI X L, XU X J, et al.. Correlation between antitumor activity,molecular weight,and conformation of lentinan[J]. Carbohydr. Res., 2005, 340(8): 1515-1521.
33	SURENJAV U, ZHANG L N, XU X J, et al.. Effects of molecular structure on antitumor activities of (1→3)-β-d-glucans from different Lentinus edodes [J]. Carbohydr. Polym., 2006, 63(1): 97-104.
34	KAMADA N, SEO S U, CHEN G Y, et al.. Role of the gut microbiota in immunity and inflammatory disease[J]. Nat. Rev. Immunol., 2013, 13:321-335.
35	YANG J, LUO J, TIAN X, et al.. Progress in understanding oxidative stress, aging,and aging-related diseases[J/OL]. Antioxidants (basel switz.), 2024, 13(4): 394[2024-10-10]. .
36	FORMAN H J, ZHANG H Q. Targeting oxidative stress in disease: promise and limitations of antioxidant therapy[J]. Nat. Rev. Drug Discov., 2021, 20: 689-709.
37	赵婷婷, 商洛华, 姚肖翰, 等. 香菇菌柄营养成分、多糖结构表征及其体外抗氧化活性[J/OL]. 菌物研究, doi:10.13341/j.‍jfr.‍2022.1522 [2024-07-08]. .
	ZHAO T T, SHANG L H, YAO X H, et al.. Lentinula edodes stalks' nutrient composition, characterization of polysaccharide structure and its antioxidant activity in vitro[J/OL]. J. Fungal Res., doi:10.13341/j.‍jfr.‍2022.1522[2024-07-08]. .
38	时国庆, 杨林霄, 李镁娟, 等. 鲜品香菇柄中多糖闪式提取工艺优化及抗氧化活性研究[J]. 食品与机械,2023, 39(8): 186-191.
	SHI G Q, YANG L X, LI M J, et al.. Study on flash extraction technology and antioxidant activity of polysaccharides from fresh Lentinus edodes stalk[J]. Food Mach., 2023, 39(8): 186-191.
39	何皎, 孙晓菲,潘琳, 等. 五种香菇营养成分及其香菇多糖的抗氧化性评价[J]. 农产品加工, 2023, (8): 56-60+65.
	HE J, SUN X F, PAN L, et al.. Evaluation of the nutritional composition of five mushroom species and the antioxidant properties of their mushroom polysaccharides[J]. Farm. Prod. Proc., 2023, (8): 56-60+65.
40	CHEN X, ZHONG H Y, ZENG J H, et al.. The pharmacological effect of polysaccharides from Lentinus edodes on the oxidative status and expression of VCAM-1mRNA of thoracic aorta endothelial cell in high-fat-diet rats[J]. Carbohydr. Polym., 2008, 74(3): 445-450.
41	YU Z H, YIN L H, QIAN Y, et al.. Effect of Lentinus edodes polysaccharide on oxidative stress,immunity activity and oral ulceration of rats stimulated by phenol[J]. Carbohydr. Polym., 2009, 75(1): 115-118.
42	CHIHARA G, MAEDA Y, HAMURO J, et al.. Inhibition of mouse sarcoma 180 by polysaccharides from Lentinus edodes (berk.) sing[J]. Nature, 1969, 222: 687-688.
43	TRIVEDI S, PATEL K, BELGAMWAR V, et al.. Functional polysaccharide lentinan: role in anti-cancer therapies and management of carcinomas[J/OL]. Pharmacol. Res. Modern Chin. Med., 2022, 2: 100045[2024-10-15]. .
44	张玉, 张琪琳, 王静林, 等. 香菇多糖构效关系、抗肿瘤作用机制及药代动力学研究进展[J]. 中国医院药学杂志, 2023, 43 (7): 804-812.
	ZHANG Y, ZHANG Q L, WANG J L, et al.. Advances in structure activity relationship, anti-tumor mechanism and pharmacokinetics of lentinan[J]. Chin. J. Hospital Pharm., 2023, 43 (7): 804-812.
45	BISEN P S, BAGHEL R K, SANODIYA B S, et al.. Lentinus edodes: a macrofungus with pharmacological activities[J]. Curr. Med. Chem., 2010, 17(22): 2419-2430.
46	WANG K P, ZHANG Q L, LIU Y, et al.. Structure and inducing tumor cell apoptosis activity of polysaccharides isolated from Lentinus edodes [J]. J. Agric. Food Chem., 2013, 61(41): 9849-9858.
47	BAO L, WANG Y, MA R, et al.. Apoptosis-inducing effects of lentinan on the proliferation of human bladder cancer T24 cells[J]. Pak. J. Pharm. Sci., 2015, 28(5): 1595-1600.
48	ZHANG Y, LIU Y, ZHOU Y X, et al.. Lentinan inhibited colon cancer growth by inducing endoplasmic reticulum stress-mediated autophagic cell death and apoptosis[J/OL]. Carbohydr. Polym., 2021, 267: 118154[2024-10-15]. .
49	LI W Y, WANG J L, HU H P, et al.. Functional polysaccharide Lentinan suppresses human breast cancer growth via inducing autophagy and caspase-7-mediated apoptosis[J]. J. Funct. Foods, 2018, 45: 75-85.
50	ZHANG Y, LI Q, WANG J F, et al.. Polysaccharide from Lentinus edodes combined with oxaliplatin possesses the synergy and attenuation effect in hepatocellular carcinoma[J]. Cancer Lett., 2016, 377(2): 117-125.
51	JIN Y, ZHANG L N, ZHANG M, et al.. Antitumor activities of heteropolysaccharides of Poria cocos mycelia from different strains and culture media[J]. Carbohydr. Res., 2003, 338(14): 1517-1521.
52	WANG K P, WANG J, LI Q, et al.. Structural differences and conformational characterization of five bioactive polysaccharides from Lentinus edodes [J]. Food Res. Int., 2014, 62: 223-232.
53	QIAN Y, WANG D, FAN M, et al.. Effects of intrinsic metal ions of lentinan with different molecular weights from Lentinus edodes on the antioxidant capacity and activity against proliferation of cancer cells[J]. Int. J. Biol. Macromol., 2018, 120: 73-81.
54	MORALES D, RUTCKEVISKI R, VILLALVA M, et al.. Isolation and comparison of α‍- and β‍-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities[J/OL]. Carbohydr. Polym., 2020, 229: 115521[2024-10-10]. .
55	XU X, YAN H, TANG J, et al.. Polysaccharides in Lentinus edodes: isolation,structure,immunomodulating activity and future prospective[J]. Crit. Rev. Food Sci. Nutr., 2014, 54(4): 474-487.
56	DAI X, STANILKA J M, ROWE C A, et al.. Consuming Lentinula edodes (shiitake) mushrooms daily improves human immunity: a randomized dietary intervention in healthy young adults[J]. J. Am. Coll. Nutr., 2015, 34(6): 478-487.
57	WANG X E, WANG Y H, ZHOU Q, et al.. Immunomodulatory effect of lentinan on aberrant T subsets and cytokines profile in non-small cell lung cancer patients[J]. Pathol. Oncol. Res., 2020, 26(1): 499-505.
58	GUO Y, CHEN X, GONG P. Classification,structure and mechanism of antiviral polysaccharides derived from edible and medicinal fungus[J]. Int. J. Biol. Macromol., 2021, 183: 1753-1773.
59	KUROKI T, LEE S, HIROHAMA M, et al.. Inhibition of influenza virus infection by Lentinus edodes mycelia extract through its direct action and immunopotentiating activity[J/OL]. Front. Microbiol., 2018, 9: 1164[2024-10-15]. .
60	REN G M, XU L M, LU T Y, et al.. Structural characterization and antiviral activity of lentinan from Lentinus edodes mycelia against infectious hematopoietic necrosis virus[J]. Int. J. Biol. Macromol., 2018, 115: 1202-1210.
61	毛湘冰, 胡海燕, 陈代文, 等. 饲粮添加香菇多糖对轮状病毒攻毒断奶仔猪回肠屏障功能的影响[J]. 动物营养学报, 2021, 33(8): 4323-4331.
	MAO X B, HU H Y, CHEN D W, et al.. Effects of dietary lentinan on ileal barrier function of weaned pigs challenged by rotavirus[J]. Chin. J. Anim. Nutr., 2021, 33(8): 4323-4331.
62	侯爱萍, 张树梅. 香菇多糖抗菌抗病毒普适性研究[J]. 药学研究, 2015, 34 (4):199-201.
	HOU A P, ZHANG S M. Study on antibacterial and antiviral universality of lentinan[J]. J. Pharm. Res., 2015, 34(4): 199-201.
63	路志芳, 蒋鹏飞. 香菇多糖的抑菌效果试验[J]. 上海蔬菜,2017(4): 85-87.
64	胡国元, 李超影, 陈默, 等. 香菇多糖和金针菇多糖的提取及其抑菌活性[J].武汉工程大学学报, 2013, 35(6): 30-34.
	HU G Y, LI C Y, CHEN M, et al.. Extraction and antimicrobial activities of polysaccharide from Lentinus edodes and Flammulina velutipes [J]. J. Wuhan Inst. Technol., 2013, 35(6): 30-34.
65	DAS L, BHAUMIK E, RAYCHAUDHURI U, et al.. Role of nutraceuticals in human health[J]. J. Food Sci. Technol., 2012, 49(2): 173-183.
66	VENTURELLA G, FERRARO V, CIRLINCIONE F, et al.. Medicinal mushrooms:bioactive compounds, use, and clinical trials[J/OL]. Int. J. Mol. Sci., 2021, 22(2): E634[2024-10-15]. .
67	AFIATI F, FIRZA S F, KUSMIATI K, et al.. The effectiveness β-glucan of shiitake mushrooms and Saccharomyces cerevisiae as antidiabetic and antioxidant in mice Sprague Dawley induced alloxan[C]//AIP Conference Proceedings. AIP Publishing, 2019, 2120.
68	SONG X, CAI W, REN Z, et al.. Antioxidant and hepatoprotective effects of acidic-hydrolysis residue polysaccharides from shiitake culinary-medicinal mushroom Lentinus edodes (Agaricomycetes) in mice[J]. Int. J. Med. Mushrooms, 2021, 23(2): 85-96.
69	IM S B, KIM K J, JIN S W, et al.. Chemical components and hepato-protective effect of Lentinula edodes fermented by lactic acid bacteria[J]. J. Mushroom, 2021, 19(3): 191-199.
70	MORALES D, TEJEDOR-CALVO E, JURADO-CHIVATO N, et al.. In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β‍-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)[J]. Food Funct., 2019, 10(11): 7325-7332.

提取方式	提取时间/min	提取温度/℃	料液比/（g·mL^-1）	多糖得率/%	参考文献
水提法	90	90	1∶10	2.01	[20]
碱提法	60	60	1∶20	18.68	[21]
酸提法	300	85	1∶40	7.49	[22]
酶-微波-超声辅助提取	10	48	1∶30	9.38	[23]
超声提取	14	室温	1∶30	14.39	[24]
亚临界水提取	15	120	1∶30	14.11	[25]
酶辅助提取	93	54	1∶29	15.65	[26]
超声强化亚临界水提取	40	140	1∶25	17.34	[18]

提取方式	提取时间/min	提取温度/℃	料液比/（g·mL^-1）	多糖得率/%	参考文献
水提法	90	90	1∶10	2.01	[20]
碱提法	60	60	1∶20	18.68	[21]
酸提法	300	85	1∶40	7.49	[22]
酶-微波-超声辅助提取	10	48	1∶30	9.38	[23]
超声提取	14	室温	1∶30	14.39	[24]
亚临界水提取	15	120	1∶30	14.11	[25]
酶辅助提取	93	54	1∶29	15.65	[26]
超声强化亚临界水提取	40	140	1∶25	17.34	[18]

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香菇多糖结构与功能研究进展

Research Progress on Structure and Function of Lentinan

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