关键词: 肺炎多糖结合疫苗, 家兔兔抗血清, 速率比浊法

Abstract: The new method of rabbit antiserum from type 1 pneumonia was established, and the type specificity and method specificity were analyzed for the determination of polysaccharide content in the preparation process of type 1 pneumococcal polysaccharide binding and conjugate vaccine. The New Zealand rabbits were immunized with designed immunization schedule．Antisera from rabbits were compared with commercial antiserum according to double immunodiffusion and ELISA results．The specificity of the antisera was assessed with rate nephelometry. The preparation method of rabbit antiserum was established, and the serum was superior to the commercial diagnostic serum, which could meet the requirements of turbidimetric type specificity and corresponding method specificity, and could be used to determine the content of polysaccharides in type 1 pneumococcal conjugates and conjugate vaccines. The results of this study provide a strong guarantee for the determination of polysaccharide content in pneumonia.

Key words: pneumonic polysaccharide conjugate vaccine, rabbit antiserum, rate nephelometry