关键词: 蕨麻多糖, IFN-&gamma, sLAG-3

Abstract: To observe the influence of PAP on the IFN-γ and soluble LAG-3 molecule level of spleen lymphocyte supernatant  in mice which acute exposured to low doses of cadmium. The solution of cadmium chloride were injected into the intraperitoneal of the test mice with 1 mg/kg. After 24 h, the model had been successfully made, PAP of low, medium and high doses were injected into the intraperitoneal for a week, spleen index and thymus index were measured at the end of the experiment, the content of IFN-γ and sLAG-3 were measured by ELISA. The cells of CD3+,CD4+,CD8+ in spleen lymphocytes was observed by immunofluorescence. The results showed that, compared with the blank control and the cadmium stained positive control, the content of IFN-γ and sLAG-3 were increased among the different doses of PAP groups. The high concentration group increased the most obvious（P<0.05）, the amount of CD3+, CD4+ cells were also correspond to the number of rising but CD8+cells were not increased significantly. It was still the most significant increase in the high dose group. PAP could increase the content of IFN-γ and soluble LAG-3 of the mice acute exposuring to low doses of cadmium, and CD3+,CD4+ lymphocytes were in a corresponding increase.

Key words: PAP, IFN-γ, sLAG-3