生物技术进展 ›› 2024, Vol. 14 ›› Issue (2): 189-195.DOI: 10.19586/j.2095-2341.2023.0120
• 进展评述 • 上一篇
收稿日期:
2023-09-25
接受日期:
2023-12-22
出版日期:
2024-03-25
发布日期:
2024-04-17
作者简介:
张春雷 E-mail: yxtkttkl@163.com
Received:
2023-09-25
Accepted:
2023-12-22
Online:
2024-03-25
Published:
2024-04-17
摘要:
随着新兴技术的不断发展,传感器和人工智能技术的应用让病原体检测更加便捷、快速和准确。然而,目前对于传感器和人工智能技术在病原体检测中的综合应用研究尚比较缺乏。对病原体检测技术进行了综述,包括传统的培养技术、分子检测技术和免疫检测技术,重点总结了基于传感器和人工智能图像识别技术的病原体检测方法,并介绍了它们各自的优势和特点,以期更清晰地了解各种病原体检测技术的优势,把握未来病原体检测技术的发展方向。
中图分类号:
张春雷. 病原微生物检测技术研究进展[J]. 生物技术进展, 2024, 14(2): 189-195.
Chunlei ZHANG. Pathogens Detection Technology: A Review[J]. Current Biotechnology, 2024, 14(2): 189-195.
1 | 国家卫生健康委员会规划发展与信息化司. 2021年我国卫生健康事业发展统计公报[J]. 中国病毒病杂志, 2022, 12(5):321-330. |
Department of Planning, Development and Informatization Technology, National Health Commission of the People's Republic of China. Statistical bulletin of China's health development in 2021[J]. Chin. J. Viral Dis., 2022, 12(5): 321-330. | |
2 | LI L, LIU Y, WU P, et al.. Influenza-associated excess respiratory mortality in China, 2010-15: a population-based study[J]. Lancet Public Health, 2019, 4(9): 473-481. |
3 | 龚慧, 申鑫, 严涵, 等. 2006-2019年中国季节性流感疾病负担估计[J]. 中华医学杂志, 2021, 101(8): 560-567. |
GONG H, SHEN X, YAN H. Estimating the disease burden of seasonal influenza in China, 2006-2019[J]. Natl. Med. J. China, 2021, 101(8): 560-567. | |
4 | WHILEY H, TAYLOR M. Legionella detection by culture and qPCR: comparing apples and oranges[J]. Crit. Rev. Microbiol., 2016, 42(1): 65-74. |
5 | ZHAO Y, LIN K, ZHANG H, et al.. Evaluation of droplet digital PCR rapid detection method and precise diagnosis and treatment for suspected sepsis (PROGRESS): a study protocol for a multi-center pragmatic randomized controlled trial[J/OL]. BMC Infect. Dis., 2022, 22(1): 630[2024-01-29]. . |
6 | 胡思宏, 游国叶. 数字PCR在新型冠状病毒检测中的应用前景[J]. 生物技术进展, 2020, 10(6): 674-679. |
HU S H, YOU G Y. Application prospects of digital PCR in detection of SARS-CoV-2[J]. Curr. Biotechnol., 2020, 10(6): 674-679. | |
7 | CHAUHAN R P, FOGEL R, LIMSON J. Overview of diagnostic methods, disease prevalence and transmission of mpox (formerly monkeypox) in humans and animal reservoirs[J/OL]. Microorganisms, 2023, 11(5): 1186[2024-01-29]. . |
8 | 胡文阳, 李朝阳, 闫晓光, 等. 非洲猪瘟病毒实时荧光定量PCR检测方法的建立[J]. 中国兽医科学,2022, 52(6): 761-766. |
HU W Y, LI Z Y, YAN X G, et al.. Establishment of qPCR detection method for African swine fever virus[J]. Chin. Vet. Sci., 2022, 52(6): 761-766. | |
9 | 杜丕波, 柳楠, 林维石, 等. 数字PCR在新型冠状病毒检测中的应用[J]. 中国消毒学杂志, 2022, 39(11): 853-858. |
DU P B, LIU N, LIN W S, et al.. Application of digital PCR in novel coronavirus detection[J]. Chin. J. Disinfect., 2022, 39(11): 853-858. | |
10 | NYARUABA R, MWALIKO C, DOBNIK D, et al.. Digital PCR applications in the SARS-CoV-2/COVID-19 era: a roadmap for future outbreaks[J/OL]. Clin. Microbiol. Rev., 2022, 35(3): e0016821[2024-01-29]. . |
11 | AL-KHALDI S F, MOSSOBA M M, ALLARD M M, et al.. Bacterial identification and subtyping using DNA microarray and DNA sequencing[J]. Meth. Mol. Biol. Clifton N. J., 2012, 881: 73-95. |
12 | CALL D R. Challenges and opportunities for pathogen detection using DNA microarrays[J]. Crit. Rev. Microbiol., 2005, 31(2): 91-99. |
13 | WILSON M R, NACCACHE S N, SAMAYOA E, et al.. Actionable diagnosis of neuroleptospirosis by next-generation sequencing[J]. N. Engl. J. Med., 2014, 370(25): 2408-2417. |
14 | GOGGIN K P, GONZALEZ-PENA V, INABA Y, et al.. Evaluation of plasma microbial cell-free DNA sequencing to predict bloodstream infection in pediatric patients with relapsed or refractory cancer[J]. JAMA Oncol., 2020, 6(4): 552-556. |
15 | ZHAO M, LI Y, GAO Z, et al.. Plasma metagenomics reveals regional variations of emerging and re-emerging pathogens in Chinese blood donors with an emphasis on human parvovirus B19[J/OL]. One Health, 2023, 17: 100602[2024-01-29]. . |
16 | LIU Y, WANG X, XU J, et al.. Diagnostic value of metagenomic next-generation sequencing of lower respiratory tract specimen for the diagnosis of suspected Pneumocystis jirovecii pneumonia[J/OL]. Ann. Med., 2023, 55(1): 2232358[2024-01-29]. . |
17 | LI Y, BIAN W, WU S, et al.. Metagenomic next-generation sequencing for Mycobacterium tuberculosis complex detection: a meta-analysis[J/OL]. Front. Public Health, 2023, 11: 1224993[2024-01-29]. . |
18 | HAN D, LI Z, LI R, et al.. mNGS in clinical microbiology laboratories: on the road to maturity[J]. Crit. Rev. Microbiol., 2019, 45(5-6): 668-685. |
19 | 李伟, 王冲, 刘嗣嘉, 等. 宏基因组学技术在痤疮研究中的应用进展[J]. 生物技术进展, 2021, 11(6): 694-699. |
LI W, WANG C, LIU S J, et al.. Application progress of metagenomic technology in acne research[J]. Curr. Biotechnol., 2021, 11(6): 694-699. | |
20 | PANG B, ZHAO C, LI L, et al.. Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157: H7 detection[J]. Anal. Biochem., 2018, 542: 58-62. |
21 | PARK S, KIM Y T, KIM Y K. Optical enzyme-linked immunosorbent assay on a strip for detection of Salmonella typhimurium [J]. BioChip J., 2010, 4(2): 110-116. |
22 | 李晓光, 陈静, 王伟, 等. 新型快速流行性感冒病毒抗原检测方法免疫荧光法在流行性感冒筛查中的应用价值研究[J]. 中国全科医学, 2020, 23(36): 4651-4655. |
LI X G, CHEN J, WANG W, et al.. Application of a novel rapid immunofluorescence assay in influenza screening[J]. Chin. Gen. Pract., 2020, 23(36): 4651-4655. | |
23 | 顾伟忠, 舒艳, 赵云, 等. 采用直接免疫荧光法对呼吸道分泌物中多种呼吸道病毒抗原检测的价值及临床应用[C]//浙江省病理技术学术大会暨第六届长三角病理技术学术会议论文汇编, 2019. |
24 | 叶夏云, 刘丽, 席春生. 单克隆抗体免疫荧光法检测孕妇生殖道沙眼衣原体[J]. 西北国防医学杂志, 2001, 22(3): 78-79. |
YE X Y, LIU L, XI C S. Detection of Chlamydia trachomatis in reproductive tract of pregnant women by monoclonal antibody immunofluorescence[J]. Med. J. Natl. Defending Forces Northwest China, 2001, 22(3): 78-79. | |
25 | 王攀, 陈贤君, 钟倩怡, 等. 三种方法在检测嗜肺军团菌中的应用和评价[J]. 中国卫生检验杂志, 2014, 24(14): 2017-2018. |
WANG P, CHEN X J, ZHONG Q Y, et al.. Application and evaluation of three methods for legionella pneumophila detection[J]. Chin. J. Health Lab. Technol., 2014, 24(14): 2017-2018. | |
26 | 王小环, 杨莲如, 赵林立, 等. 免疫荧光检测技术及其在寄生虫检测中的应用进展[J]. 中国畜牧兽医, 2012, 39(3): 81-84. |
WANG X H, YANG L R, ZHAO L L, et al.. Brief introduction of immunefluorescent test technology and application progress on parasites detection[J]. China Anim. Husb. Vet. Med., 2012, 39(3): 81-84. | |
27 | 张荣, 李雪波, 王玲, 等. 免疫荧光法检测生殖道病原体感染的临床研究[J]. 中国社区医师, 2023, 39(19): 90-92. |
ZHANG R, LI X B, WANG L, et al.. Clinical study on immunofluorescence assay for detecting reproductive tract pathogen infections[J]. Chin. Commun. Doctors, 2012, 39(3): 81-84. | |
28 | MANSFIELD L P, FORSYTHE S J. The detection of Salmonella using a combined immunomagnetic separation and ELISA end-detection procedure[J]. Lett. Appl. Microbiol., 2000, 31(4): 279-283. |
29 | PENG H, SHELEF L A. Automated rapid screening of foods for the presence of salmonellae[J]. J. Food Prot., 1999, 62(11): 1341-1345. |
30 | WATTAM A R, INZANA T J, WILLIAMS K P, et al.. Comparative genomics of early-diverging Brucella strains reveals a novel lipopolysaccharide biosynthesis pathway[J/OL]. mBio, 2012, 3(5): 00246-12[2023-12-24]. . |
31 | 黄震, 罗梅霞, 汪泽祥, 等. 免疫磁分离法高效富集牛奶中大肠杆菌O157: H7[J]. 食品安全质量检测学报,2019,10(14):4492-4497. |
HUANG Z, LUO M X, WANG Z X, et al.. Efficient enrichment of Escherichia coli O157: H7 in milk based on immunomagnetic separation[J]. J. Food Saf. Qual., 2019, 10(14): 4492-4497. | |
32 | 林吉恒, 黄朱梁, 彭志兰, 等. 免疫磁珠分离技术在食源性致病菌检测中的应用[J]. 食品安全质量检测学报, 2019, 10(18): 5998-6005. |
LIN J H, HUANG Z L, PENG Z L, et al.. Application of immunomagnetic beads separation techniques in detection of foodborne pathogenic bacteria[J]. J. Food Saf. Qual., 2019, 10(18): 5998-6005. | |
33 | HO J A A, HSU H W, HUANG M R. Liposome-based microcapillary immunosensor for detection of Escherichia coli O157: H7[J]. Anal. Biochem., 2004, 330(2): 342-349. |
34 | WANG S, XIE J, JIANG M, et al.. The development of a portable SPR bioanalyzer for sensitive detection of Escherichia coli O157: H7[J/OL]. Sensors, 2016, 16(11): 1856[2024-01-29]. . |
35 | BANAKAR M, HAMIDI M, KHURSHID Z, et al.. Electrochemical biosensors for pathogen detection: an updated review[J/OL]. Biosensors, 2022, 12(11): 927[2024-01-29]. . |
36 | FERNANDES A M, ABDALHAI M H, JI J, et al.. Development of highly sensitive electrochemical genosensor based on multiwalled carbon nanotubes-chitosan-bismuth and lead sulfide nanoparticles for the detection of pathogenic Aeromonas [J]. Biosens. Bioelectron., 2015, 63: 399-406. |
37 | FLOW K, ZAIN Z M, YEAN C Y. A signal-amplified electrochemical DNA biosensor incorporated with a colorimetric internal control for Vibrio cholerae detection using shelf-ready reagents[J]. Biosens. Bioelectron., 2017, 87: 256-263. |
38 | SUN Y, HE X, JI J, et al.. A highly selective and sensitive electrochemical CS-MWCNTs/Au-NPs composite DNA biosensor for Staphylococcus aureus gene sequence detection[J]. Talanta, 2015, 141: 300-306. |
39 | CIMAFONTE M, FULGIONE A, GAGLIONE R, et al.. Screen printed based impedimetric immunosensor for rapid detection of Escherichia coli in drinking water[J/OL]. Sensors, 2020, 20(1): 274[2024-01-29]. . |
40 | ABDELRASOUL G N, ANWAR A, MACKAY S, et al.. DNA aptamer-based non-faradaic impedance biosensor for detecting E. coli [J]. Anal. Chim. Acta, 2020, 1107: 135-144. |
41 | GOSWAMI N, HE Y R, DENG Y H, et al.. Label-free SARS-CoV-2 detection and classification using phase imaging with computational specificity[J/OL]. Light Sci. Appl., 2021, 10(1): 176[2024-01-29]. . |
42 | RAHMAN M A, CLINCH M, REYNOLDS J, et al.. Classification of fungal Genera from microscopic images using artificial intelligence[J/OL]. J. Pathol. Inform., 2023, 14: 100314[2024-01-29]. . |
43 | TAO C, DU J, TANG Y, et al.. A deep-learning based system for rapid genus identification of pathogens under hyperspectral microscopic images[J/OL]. Cells, 2022, 11(14): 2237[2024-01-29]. . |
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