滚环扩增技术在电化学生物传感器中的应用

doi:10.19586/j.2095-2341.2023.0122

生物技术进展 ›› 2023, Vol. 13 ›› Issue (6): 863-867.DOI: 10.19586/j.2095-2341.2023.0122

• 进展评述 • 上一篇

滚环扩增技术在电化学生物传感器中的应用

杨玉琦(), 何秀霞()

长春理工大学生命科学技术学院，长春 130022

收稿日期:2023-10-12 接受日期:2023-11-13 出版日期:2023-11-25 发布日期:2023-12-12
通讯作者: 何秀霞
作者简介:杨玉琦 E-mail:13844638909@163.com；
基金资助:
吉林省科技厅项目(20210204009YY);吉林省教育厅项目(JJKH20210853KJ)

Application of Rolling Circle Amplification Technique in Electrochemical Biosensors

Yuqi YANG(), Xiuxia HE()

School of Life Science and Technology，Changchun University of Science and Technology，Changchun 130022，China

Received:2023-10-12 Accepted:2023-11-13 Online:2023-11-25 Published:2023-12-12
Contact: Xiuxia HE

摘要/Abstract

摘要：

在电化学生物传感器的设计中，信号放大是实验环节中的重要步骤，特别是对靶标进行灵敏度分析时更是不可或缺。滚环扩增（rolling circle amplification，RCA）能够在短时间内得到大量产物，并在电极表面进行扩增或孵育，然后通过一定的设计使电化学信号被快速放大。RCA技术具有高度的灵敏性和特异性，电化学生物传感器则可提供实时、快速、低成本的检测。为了更好的了解RCA，介绍了RCA环化的基本原理、RCA种类，重点总结了RCA与电化学生物传感器结合的不同技术类型及应用，并对未来相关研究领域的发展趋势进行了展望，旨在为RCA技术在电化学生物传感器中的进一步发展和应用提供参考。

关键词: 滚环扩增技术, 快速检测, 等温扩增, 电化学生物传感器

Abstract:

In the design of electrochemical biosensors， signal amplification is an important step in the experimental process， especially when analyzing the sensitivity of the target. Rolling circle amplification （RCA） could obtain a large amount of products in a short period of time and amplify or incubate them on the electrode surface. Through certain design， the electrochemical signal can be quickly amplified. RCA technology has high sensitivity and specificity， while electrochemical biosensors can provide real-time， fast， and low-cost detection. In order to better understand RCA， the basic principle and types of RCA cyclization were introduced， with a focus on summarizing the different technical types and applications of RCA combined with electrochemical biosensors. The development trend of future related research fields was also prospected， aiming to provide reference for the further development and application of RCA technology in electrochemical biosensors.

Key words: rolling circle amplification, rapid detection, isothermal amplification, electrochemical biosensors

中图分类号:

Q503

杨玉琦, 何秀霞. 滚环扩增技术在电化学生物传感器中的应用[J]. 生物技术进展, 2023, 13(6): 863-867.

Yuqi YANG, Xiuxia HE. Application of Rolling Circle Amplification Technique in Electrochemical Biosensors[J]. Current Biotechnology, 2023, 13(6): 863-867.

图/表 1

参考文献 22

1	GRIESHABER D, MACKENZIE R, VÖRÖS J, et al.. Electrochemical biosensors-sensor principles and architectures[J]. Sensors, 2008, 8(3): 1400-1458.
2	MAITY S, SAHU P P. Electrochemical sensors: core principle, new fabrication trends, and their applications[M]. Biosensors in food safety and quality: fundamentals and applications. 2022: 47-62.
3	XU L, DUAN J, CHEN J, et al.. Recent advances in rolling circle amplification-based biosensing strategies—a review[J/OL]. Anal. Chim. Acta, 2021, 1148: 238187[2023-11-10]. .
4	SOARES R R G, MADABOOSI N, NILSSON M. Rolling circle amplification in integrated microsystems: an uncut gem toward massively multiplexed pathogen diagnostics and genotyping[J]. Acc. Chem. Res., 2021, 54(21): 3979-3990.
5	WANG J, ZHOU H, LIU J, et al.. Electrochemical detection of DNA by formation of efficient electron transfer pathways through adsorbing gold nanoparticles to DNA modified electrodes[J/OL]. Microchem. J., 2021, 169: 106581[2023-11-10]. .
6	WANG Y, WANG X, GAILING O, et al.. Visual detection of Fusarium proliferatum based on dual-cycle signal amplification and T5 exonuclease[J]. RSC Adv., 2020, 10(58): 35131-35135.
7	OU L J, SUN A M, LIU K J. Rolling circle amplification-based biosensors[J]. Anal. Lett., 2015, 48(8): 1199-216.
8	占忠旭,刘巨,陈博璐,等.滚环扩增信号放大技术在生物检测中应用的研究进展[J].生物工程学报,2019,35(7):1206-1213.
9	TIAN W, LI P, HE W, et al.. Rolling circle extension-actuated loop-mediated isothermal amplification (RCA-LAMP) for ultrasensitive detection of microRNAs[J]. Biosens. Bioelectron., 2019, 128: 17-22.
10	WU D, YI X, XIA N. Electrochemical biosensors for microRNA detection using duplex-specific nuclease based signal amplification strategies[J]. Int. J. Electrochem. Sci., 2020, 15(12): 12136-12148.
11	WEI H, BU S, ZHANG W, et al.. An electrochemical biosensor for the detection of pathogenic bacteria based on dual signal amplification of Cu₃(PO₄)₂-mediated click chemistry and DNAzymes[J]. Analyst, 2021, 146(15): 4841-4847.
12	HAJIAN R, TAYEBI Z, SHAMS N. Fabrication of an electrochemical sensor for determination of doxorubicin in human plasma and its interaction with DNA[J]. J. Pharm. Anal., 2017, 7(1): 27-33.
13	ZHAN X, YANG S, HUANG G, et al.. Streptavidin-functionalized terahertz metamaterials for attomolar exosomal microRNA assay in pancreatic cancer based on duplex-specific nuclease-triggered rolling circle amplification[J/OL]. Biosens. Bioelectron., 2021, 188: 113314[2023-11-11]. .
14	CHEN H, WU S, DONG F, et al.. A novel chemiluminescence immunoassay for highly sensitive and specific detection of protein using rolling circle amplification and the multiplex binding system[J]. Sens. Actuat. B Chem., 2015, 221: 328-333.
15	XIE Y, DU J, LIU Z, et al.. miR-6875-3p promotes the proliferation, invasion and metastasis of hepatocellular carcinoma via BTG2/FAK/Akt pathway[J/OL]. J. Exp. Clin. Cancer Res., 2019, 38(1): 7[2023-11-10]. .
16	HUANG R, HE L, XIA Y, et al.. A sensitive aptasensor based on a hemin/G-quadruplex-assisted signal amplification strategy for electrochemical detection of gastric cancer exosomes[J/OL]. Small, 2019, 15(19): e1900735[2023-11-11]. .
17	DING C, WANG N, ZHANG J, et al.. Rolling circle amplification combined with nanoparticle aggregates for highly sensitive identification of DNA and cancer cells[J]. Biosens. Bioelectron., 2013, 42: 486-491.
18	SHENG Q, CHENG N, BAI W, et al.. Ultrasensitive electrochemical detection of breast cancer cells based on DNA-rolling-circle-amplification-directed enzyme-catalyzed polymerization[J]. Chem. Commun. Camb. Engl., 2015, 51(11): 2114-2117.
19	ZHAN Z, LI H, LIU J, et al.. A competitive enzyme linked aptasensor with rolling circle amplification (ELARCA) assay for colorimetric detection of Listeria monocytogenes [J/OL]. Food Contr., 2020, 107: 106806[2023-11-10]. .
20	XU J, GUO J, MAINA S W, et al.. An aptasensor for staphylococcus aureus based on nicking enzyme amplification reaction and rolling circle amplification[J]. Anal. Biochem., 2018, 549: 136-142.
21	TANG S, TONG P, LI H, et al.. Ultrasensitive electrochemical detection of Pb²⁺ based on rolling circle amplification and quantum dots tagging[J]. Biosens. Bioelectron., 2013, 42: 608-611.
22	QING M, YUAN Y, CAI W, et al.. An ultrasensitive electrochemical biosensor based on multifunctional hemin/G-quadruplex nanowires simultaneously served as bienzyme and direct electron mediator for detection of lead ion[J]. Sens. Actuat. B Chem., 2018, 263: 469-475.

[1]	王腾, 余逸飞, 王睿, 王鑫, 李昀怡, 陈洋, 程楠. 食品中邻苯二甲酸酯类塑化剂检测方法研究进展[J]. 生物技术进展, 2023, 13(1): 11-21.
[2]	刘旺, 靳晶豪, 陈孝仁. 环介导等温扩增技术的应用进展[J]. 生物技术进展, 2021, 11(2): 128-135.
[3]	瞿展,贾犇,杨立桃. 普通牛及其近缘种特异性序列筛选及环介导等温扩增检测方法[J]. 生物技术进展, 2020, 10(6): 704-710.
[4]	纪艺,陈笑芸,丁霖,汪小福,彭城,徐晓丽,徐俊锋. 肉类及肉制品中动物源性成分鉴别方法研究进展[J]. 生物技术进展, 2020, 10(6): 711-716.
[5]	马翾，高思悦，李可馨，梁志宏，姚志轶. 硫酸软骨素荧光传感器的构建新策略[J]. 生物技术进展, 2020, 10(2): 164-169.
[6]	林晟豪,杜再慧,张秀杰,黄昆仑,,刘清亮4,许文涛,. 基于环介导等温扩增技术的生物传感器研究进展[J]. 生物技术进展, 2019, 9(6): 599-610.
[7]	彭志,陈刚毅,刘雪飞,黄新河. 等温核酸扩增技术在病原体检测中的应用[J]. 生物技术进展, 2018, 8(4): 284-292.
[8]	张捷,王煜,陆琳,刘艳华,周琦,孙梓晏,顾德周,尚世进,张惠媛,王佩荣,陈广全,乐加昌. F₀F₁-ATPase分子马达技术对阪崎肠杆菌检测的研究[J]. 生物技术进展, 2013, 3(4): 297-300.
[9]	张捷,张昕,范爱红,张惠媛,汪琦,顾德周,王佩荣,韩晶,陆琳,陈广全,乐加昌. F₀F₁-ATPase分子马达技术对单核细胞增生李斯特菌检测的研究[J]. 生物技术进展, 2012, 2(6): 436-440.

滚环扩增技术在电化学生物传感器中的应用

Application of Rolling Circle Amplification Technique in Electrochemical Biosensors

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 1

参考文献 22

相关文章 9

编辑推荐

Metrics

本文评价