关键词: 等温核酸扩增, 依赖核酸序列扩增, 环介导等温扩增, 重组酶聚合酶扩增, 病原体检测技术

Abstract: In recent years, new infectious diseases, such as severe acute respiratory syndrome (SARS) and H7N9 avian influenza, have outbroken one after another, which seriously threatened human health and the sustainable development of society and economy, and brought severe challenges to the prevention and control of infectious diseases. Pathogen detection is an important link in the prevention and control of infectious diseases, and therefore more and more new techniques have been applied in it. As a rapid and high sensitivity pathogen detection technology, isothermal nucleic acid amplification technology has obtained the considerable development. The principle, important characteristics and application progress in pathogen detection of isothermal nucleic acid amplification technique were reviewed, which were expected to provide important references for the promotion and application of this valuable technique.

Key words: isothermal nucleic acid amplification, nucleic acid sequence-based amplification, loop-mediated isothermal amplification, recombinant enzyme dependent polymerase amplification, pathogen detection techniques