关键词: 环介导等温扩增技术, 核酸扩增, 生物技术, 检测, 植物病害, 应用

Abstract: Loop-mediated isothermal amplification (LAMP) is a new nucleic acid amplification technology, which relies on an active DNA polymerase with chain replacement activity and two pairs of specially designed primers to complete the amplification reaction efficiently and quickly under isothermal conditions. Compared with traditional amplification detection methods, LAMP technology has the advantages of strong specificity, high sensitivity, simple and rapid operation, and can be widely popularized in field rapid detection and grass-roots application. At present, LAMP technology has been widely used in the detection of plant diseases, animal diseases, food safety and other fields. Based on this, the basic principle of LAMP technology and the detection method of reaction products was briefly introduced, the improvement and development of LAMP technology was emphatically expounded, its application progress in scientific research and production in recent years was summarized, and its development prospect was looked forward, in order to provide a reasonable research direction for the further development of LAMP technology.

Key words: loop-mediated isothermal amplification, nucleic acid amplification, biological technique, detection, plant disease, application