关键词: 硫酸软骨素, 芘, 快速检测, 荧光传感器

Abstract: Chondroitin sulfate (CS) is a kind of protein viscose polysaccharide extracted from animal tissues, which plays a role in immunomodulation, relieving joint pain, anticoagulant activity and protecting neurons. It is of great significance to develop CS sensor for the coarse screening of the relevant pharmaceutical and health care products in the biomedical industry. In this paper, based on the characteristics of simple preparation, rapid response and strong selectivity of fluorescent sensors, a cationic polymer PDDA was used to induce the self-assembly of the pyrene-based molecule HPTS, resulting in fluorescence quenching to construct the sensor. The addition of the analyte caused the recovery of the fluorescence intensity at 510 nm. We finally established a rapid analysis method for CS in the actual sample. The method showed a good linear relationship at a concentration of 0～5 μmol·L-1. The linear equation was y=1.907 52+0.273 59x (R2=0.998 2), the detection limit was 2.8 μmol·L-1, and the sample recovery was 104%～121%. This sensor features good water solubility, strong specificity and intuitive visualization.

Key words: chondroitin sulfate, pyrene, rapid detection, fluorescence quenching