生物技术进展 ›› 2016, Vol. 6 ›› Issue (5): 319-327.DOI: 10.3969/j.issn.2095-2341.2016.05.03

• 进展评述 • 上一篇    下一篇

生物膜定量分析方法研究进展

赵智颖,李良秋*,马连营,游雪娇   

  1. 广东省微生物研究所, 省部共建华南应用微生物国家重点实验室; 广东省菌种保藏与应用重点实验室, 广州 510070
  • 收稿日期:2016-06-02 出版日期:2016-09-25 发布日期:2016-06-30
  • 通讯作者: 李良秋,教授级高级工程师,研究方向为微生物资源与发酵工程。E-mail: liliangqiu1010@163.com
  • 作者简介:赵智颖,助理工程师,研究方向为微生物资源学。 E-mail:crystal23200@163.com。
  • 基金资助:
    广东省科学院分析测试基金项目(Sf201502)资助。

Review on the Quantification Analysis Methods of Biofilm

ZHAO Zhi-ying, LI Liang-qiu, MA Lian-ying, YOU Xue-jiao   

  1. State Key Laboratory of Applied Microbiology Southern China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China
  • Received:2016-06-02 Online:2016-09-25 Published:2016-06-30

摘要: 生物膜因具有强致病性、耐药性和抗逆性,已成为全球关注的重大难题。介绍了生物膜生物量、细胞总数、活细胞数、大分子物质和结构的定量分析方法,以期为深入研究生物膜提供更丰富的手段,推动生物膜定量分析方法的改进与提高,从而更好地预防、控制及开发生物膜。

关键词: 生物膜, 定量, 检测

Abstract: Because of strong pathogenicity, drug resistance and stress resistance, biofilm has become a major problem of global concern. In this paper, a review was made on the quantitative analysis methods of biofilm biomass, total cell count, living cell number, macromolecular substance and structure, which was expected to provide abandant methods for biofilm research. We are also looking forward to provide theory reference to promote biofilm quantitative analysis method, thereby we can better prevent, control and exploit biofilm.

Key words: biofilm, quantification, detection