生物技术进展 ›› 2012, Vol. 2 ›› Issue (2): 92-97.DOI: 10.3969/j.issn.2095-2341.2012.02.03

• 进展评述 • 上一篇    下一篇

短短芽胞杆菌功能基因的研究及其应用

马桂美1,2,车建美1,刘波1*,史怀1,陈峥1   

  1. 1.福建省农业科学院农业生物资源研究所, 福州 350003;
    2.福建农林大学生物农药与化学生物学教育部重点实验室, 福州 350002
  • 收稿日期:2012-01-13 出版日期:2012-03-25 发布日期:2012-02-27
  • 通讯作者: 刘波,研究员,博士,研究方向为微生物生物技术与农业生物药物。E-mail:liubofaas@163.com
  • 作者简介:马桂美,硕士研究生,研究方向为微生物分子生物学。E-mail:mgm658743@163.com。
  • 基金资助:

    973计划前期研究专项(2011CB111607);农业部引进国际先进农业科学技术重点项目(2011-G25);公益性行业(农业)科研专项(200903034);福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y03);中国热带农业科学院院本级中央级公益性科研院所基本科研业务费专项和福建省产业技术开发项目(闽发改高技\[2010\]1002号)资助。

Progress on Functional Genes of Brevibacillus brevis and its Application

MA Gui-mei, CHE Jian-mei, LIU Bo, SHI Huai, CHEN Zheng   

  1. 1.Agricultural Bio-resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China;
    2.Key Laboratory of Biopesticide and Chemical Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2012-01-13 Online:2012-03-25 Published:2012-02-27

摘要: 短短芽胞杆菌分布广泛,并且在不同生长阶段,菌落形态也有所不同。目前已经完成2株短短芽胞杆菌的全基因组测序,但只对短短芽胞杆菌部分基因的功能进行了研究,如编码二硫化物氧化还原酶的基因bdb、α-乙酰乳酸脱羧酶的基因aldB、耐碱性木聚糖酶xylB基因和细胞壁的合成基因等,特别是具有抗菌活性的短杆菌酪肽的合成过程及所需酶的基因均有研究。短短芽胞杆菌在生物防治、作为分泌表达外源蛋白的宿主及环境治理等领域有广泛应用前景。

关键词: 短短芽胞杆菌, 基因, 微生物降解, 生物防治

Abstract: Brevibacillus brevis is widely distributed. In different growth stages, the colony morphology would present diversity. At present, the whole genomic sequences of two B. brevis strains were sequenced, only a number of functional genes of B. brevis have been studied, such as bdb gene which encoded thiol-disulfide oxidoreductase,aldB gene which encoded α-acetolactate decarboxylase,xylB gene which encoded alkaline xylanase and the synthesis of cell wall genes. Genes expressed in tyrocidine synthesis process and its required enzymes have also been studied. B. brevis was used in lots of fields, such as biological control, as host bacteria for extracellular production of foreign proteins, and microbial degradation etc, it wonld have broad application prospects.

Key words: Brevibacillus brevis, gene, microbial degradation, biocontrol