关键词: 谷子品种, 2, 4-D浓度, 干燥处理, 正交实验法

Abstract: In vitro regeneration system of foxtail millet is not stable and its transformation efficiency is low, which has become the bottleneck of functional gene research and variety improvement of foxtail millet. In order to establish a stable in vitro regeneration system for mature millet embryos, the mature embryos of 6 local foxtail millet varieties with high yield and good quality were used as explants, and the effects of different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and different desiccation treatment time on embryogenic callus were investigated by single factor and orthogonal experiment  on callus differentiation and seedling formation of foxtail millet. The results showed that the best regeneration system was established under the conditions of Jingu21, 2,4-D concentration of 9 μmol·L-1 and 4 h desiccation treatment. Under these conditions, the differentiation rate was 64.35%, and the seedling formation rate was 29.06%. The study provided a basis for the establishment of efficient and stable genetic transformation of millet and the quality improvement by means of genetic engineering by exploring the optimum conditions for tissue culture of millet.

Key words: foxtail millet varieties, 2,4-D concentrations, desiccation, orthogonal experiment method