生物技术进展 ›› 2022, Vol. 12 ›› Issue (4): 630-637.DOI: 10.19586/j.2095-2341.2021.0145

• 研究论文 • 上一篇    

异养硝化⁃好氧反硝化复合菌剂在垃圾渗滤液处理中的应用

高宇轩1,2(), 靳静晨3, 高雅娟1,2, 张闻天1,2, 李晨晨1,2, 靳永胜1,2()   

  1. 1.北京农学院生物与资源环境学院,北京 102206
    2.农业农村部华北都市农业重点实验室,北京 102206
    3.北京环氧环保科技发展有限公司,北京 100028
  • 收稿日期:2021-08-30 接受日期:2021-10-28 出版日期:2022-07-25 发布日期:2022-08-10
  • 通讯作者: 靳永胜
  • 作者简介:高宇轩 E-mail:1031836790@qq.com

Application of Heterotrophic Nitrification Aerobic Denitrifying Bacteria in the Landfill Leachate Treatment

Yuxuan GAO1,2(), Jingchen JIN3, Yajuan GAO1,2, Wentian ZHANG1,2, Chenchen LI1,2, Yongsheng JIN1,2()   

  1. 1.College of Bioscience and Resources Environment,Beijing University of Agriculture,Beijing 102206,China
    2.Key Laboratory of Urban Agriculture in North China,Ministry of Agriculture and Rural Affairs,Beijing 102206,China
    3.Beijing Epoxy Environmental Protection Technology Development Co. Ltd. ,Beijing 100028,China
  • Received:2021-08-30 Accepted:2021-10-28 Online:2022-07-25 Published:2022-08-10
  • Contact: Yongsheng JIN

摘要:

垃圾渗滤液中往往含有高浓度的有机物、氨氮等污染物。异养硝化-好氧反硝化型微生物能在脱氮的同时去除部分有机物,但目前对于相关混合菌剂直接应用于垃圾渗滤液处理的研究较少。从垃圾渗滤液中筛选出6株异养硝化-好氧反硝化菌株并组配成复合菌剂F6,探究菌剂在垃圾渗滤液中的脱氮效果。分别以单一菌株和复合菌剂F6为投放原料;以不同碳氮比、活性炭浓度、转速以及微量元素浓度为影响因素,研究复合菌剂对于垃圾渗滤液中氨氮(NH4+-N)、总氮(total nitrogen, TN)、化学需氧量(chemical oxygen demand, COD)的去除性能。结果表明,提高碳氮比和微量元素浓度能够促进复合菌剂F6的降解效果;当接种量为10%、碳氮比为15%时,F6对NH4+-N、TN、COD的去除率分别为74.69%、89.23%和83.50%,与不添加活性炭的处理相比,分别提高了约18.46%、20.97%和7.98%。复合异养硝化-好氧反硝化菌剂F6对高氨氮垃圾渗滤液去除NH4+-N、TN、COD等方面具有良好的应用前景。

关键词: 异养硝化-好氧反硝化, 垃圾渗滤液, NH4+-N, TN, COD, 生物脱氮

Abstract:

Landfill leachate often contains high concentration of organic matter, ammonia nitrogen and other pollutants. Heterotrophic nitrification-aerobic denitrification-type microorganisms can remove some organic matter while removing nitrogen, but there are few studies on the application of related mixed bacteria directly to landfill leachate treatment. In this study, six strains of heterotrophic nitrification-aerobic denitrification were screened out from landfill leachate and combined into a compound bacterial agent F6, to explore the nitrogen removal effect of the bacterial agent in landfill leachate. Single strain and compound bacterial agent F6 were used as raw materials. Taking different C/N ratios, concentration of activated carbon, rotational speed and concentration of trace elements as influencing factors, the removal performance of compound microbial agent for ammonia nitrogen (NH4+-N), total nitrogen (TN) and chemical oxygen demand (COD) in landfill leachate was studied. The results showed that the degradation of F6 could be promoted by increasing the C/N ratio and the concentration of trace elements. When the inoculation amount was 10% and the C/N ratio was 15%, the removal rates of NH4+-N, TN and COD by F6 were 74.69%, 89.23% and 83.50%, respectively, which increased by 18.46%, 20.97% and 7.98%, respectively, compared with the treatment without activated carbon. Compound heterotrophic nitrification-aerobic denitrification bacterial agent F6 has a good application prospect in the removal of NH4+-N, TN, COD and other aspects of high ammonia nitrogen landfill leachate.

Key words: heterotrophic nitrification aerobic denitrification, landfill leachate, NH4+-N, TN, COD, biological denitrification

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