关键词: 椎间盘退变, IL-13, 胶原蛋白, sIL-13R&alpha, 2-Fc

Abstract: To explore the effect of interleukin-13 signaling pathway on intervertebral disc degeneration in rats, we established a rat model of degeneration of the tail intervertebral disc. The experiment was divided into blank, control, low-dose, middle-dose, and high-dose intervention groups. The morphological changes of intervertebral discs by HE staining and Masson staining was observed and scored. Quantitative analysis of glycosaminoglycan, chondroitin sulfate, keratan sulfate, and hyaluronic acid content changes was analyzed in intervertebral discs by DMMB method. mRNA expression levels of typeⅠ and typeⅡ collagen was analyzed by RT-PCR analysis. TypeⅠand typeⅡ collagen content was analyzed by western blot analysis. HE and Masson staining results showed that the pathological changes of the intervertebral disc were reduced compared with the control group, the fibrous annulus arrangement was more regular, the rupture site was reduced, the number of NP cells was increased, and the collagen fibers were reduced. sIL-13Rα2-Fc intervention increased GAG and HA content, Ⅰ collagen and the CS/KS ratio, and decreased Ⅱ collagen content. These results indicated that sIL-13Rα2-Fc can effectively reduce the degeneration of the intervertebral disc, and it is positively correlated with the action time and concentration.

Key words: intervertebral disc degeneration, interleukin-13, collagen, sIL-13Rα2-Fc