关键词: 腹膜炎模型, 血小板, RNA-Seq, 黏附分子

Abstract: As the smallest non-nucleated blood cell, platelets play  important roles in regulating innate and adaptive immunity beyond the classic hemostatic function. However, the molecular mechanism of platelets in immune regulation remains relatively understudied. The mouse peritonitis model was constructed by injecting heat-inactivated Escherichia coli (E. coli) into the abdominal cavity. The dynamics of peritoneal immune cells and peripheral blood cells during acute inflammations were also detected by using blood routine, flow cytometry and other experimental methods. It was found that in the early stage of infection, neutrophils in the peripheral blood could quickly migrate to the abdominal cavity to participate in the inflammatory response, and the number and size of platelets were also changed significantly. The significant changes in the platelet transcriptome after infection were further revealed by RNA sequencing. Strikingly, the immune response-related genes, as well as the adhesion molecules in platelets were remarkably up-regulated. This study revealed the dramatic changes of platelet transcriptome under inflammatory conditions, providing a new perspective for understanding the underlying molecular mechanism of platelets in immune regulation.

Key words: peritonitis model, platelets, RNA sequencing, adhesion molecules