关键词: 高通量测序, RNA-Seq, 果树, 次生代谢产物

Abstract: RNA-Seq, also called "Transcriptome Sequencing Technology", can be used to study the species gene function and genetic structure at an overall level to reveal specific biological processes. The application of this technique can effectively resolve the issue of huge genome of fruit trees and other problems, and efficaciously reveal and elucidate biosynthetic pathways and metabolic mechanisms of secondary metabolism in the plant. This article mainly discussed the application method and processes of the RNA-Seq technology; We analized in-depth the synthesis mechanisms of rich small molecular compounds with various secondary bioactive metabolites in synthetic fruit trees and carried out researches on the key enzyme genes synthesizing this pathway. The high-throughput sequencing technology based on RNA-Seq makes it possible to discover and reveal the key genes and regulation and control mechanisms for the synthesis of secondary metabolites with important biological functions and economic values.

Key words: high throughput sequencing, RNA-Seq, fruit tree, secondary metabolite