关键词: 琥珀酸, 大肠杆菌, 代谢网络, 基因表达调控

Abstract: Succinic acid, an important C4 compound, is often used as a raw material for synthesis of commodity chemicals. It has broad application prospects in chemical industry, food, medicine, pesticide, etc. Compared with the traditional chemical synthesis, microbial fermentation production of succinic acid is gradually considered as  focus in the world due to its renewable and environment-friendly nature. This paper reviewed the research progress of how to improve succinic acid production rate of Escherichia coli(E. coli) by regulating metabolic pathway in recent years.  We also  summerized the metabolic network of succinic acid, the regulation on gene of the key enzymes in metabolic pathway and the influence of the production of succinic acid caused by the ratio of NADH/NAD+. In addition, we put forward a suggestion that reform the succinic acid metabolic pathways in E. coli by RNAi and gave a new idea about constructing a genetic engineering strain for improving the production of succinic acid.

Key words: succinic acid, E. coli, metabolic network, regulation of gene expression