远志皂苷与莲心碱对七氟烷诱发新生大鼠急性和远期认知功能损伤的改善作用及机制分析

doi:10.19586/j.2095-2341.2021.0013

生物技术进展 ›› 2021, Vol. 11 ›› Issue (6): 807-812.DOI: 10.19586/j.2095-2341.2021.0013

远志皂苷与莲心碱对七氟烷诱发新生大鼠急性和远期认知功能损伤的改善作用及机制分析

韩丽丽(), 王惠霞(), 兰新昂, 韩慧梅, 孙洪超

中国人民解放军联勤保障部队第九六〇医院麻醉科，济南 250031

收稿日期:2021-01-27 接受日期:2021-04-25 出版日期:2021-11-25 发布日期:2021-11-26
通讯作者: 王惠霞
作者简介:韩丽丽 E-mail: chenxqqa@163.com；

Analysis of Improvement Effect and Mechanism of Tenuigenin and Liensinine on Sevoflurane‑induced Acute and Longterm Cognitive Impairments of Neonatal Rats

Lili HAN(), Huixia WANG(), Xinang LAN, Huimei HAN, Hongchao SUN

Anesthesia Department，the 960th Hospital of the PLA Joint Logistice Support Force，Jinan 250031，China

Received:2021-01-27 Accepted:2021-04-25 Online:2021-11-25 Published:2021-11-26
Contact: Huixia WANG

摘要/Abstract

摘要：

为了分析远志皂苷与莲心碱对七氟烷诱发新生大鼠急性和远期认知功能损伤的改善作用及机制，将36只新生SD大鼠随机分为中药组（n=12）、模型组（n=12）和空白组（n=12），中药组、模型组暴露于2.5%七氟烷环境中麻醉诱导2 h·d^-1，连续3 d，空白组暴露在不含七氟烷的相同环境中，中药组于麻醉前30 min腹腔注射远志皂苷与莲心碱1:1配伍溶液（30 mg·kg^-1），模型组和空白组腹腔注射等量0.9%氯化钠溶液，比较各组大鼠血气指标、皮质脑电图结果、Morris水迷宫行为和血清皮质酮含量，并采用Western blot法检测海马Bcl₂、Bax和Caspase?3表达水平。中药组大鼠皮质脑电图癫痫波总时长、平均时长以及数量均低于模型组（P<0.05）；定位航行实验结果显示，中药组大鼠逃避潜伏期短于模型组（P<0.05）；空间探索实验结果显示，中药组大鼠探索时间长于模型组（P<0.05）；中药组大鼠血清皮质酮含量低于模型组（P<0.05）；中药组大鼠海马组织Bcl₂/Bax表达水平高于模型组（P<0.05），cleaved Caspase?3表达水平低于模型组（P<0.05）。结果表明，远志皂苷与莲心碱可改善七氟烷诱发新生大鼠急性和远期认知功能损伤，其机制可能与调控凋亡相关基因Bcl₂、Bax和Caspase?3表达水平有关。

关键词: 七氟烷, 远志皂苷, 莲心碱, Bcl₂, Bax, Caspase?3

Abstract:

The aim of this study was to analyze the improvement effect and mechanism of tenuigenin and liensinine on sevofluraneinduced acute and longterm cognitive impairments of neonatal rats. 36 neonatal SD rats were randomly divided into TCM （traditional Chinese medicine） group （n=12）， model group （n=12） and blank group （n=12）. TCM group and model group were exposed to 2.5% sevoflurane environment for 2 h·d^-1 of anesthesia induction and lasted for 3 days， and blank group was exposed to the same environment without sevoflurane. TCM group was intraperitoneally injected with a 1：1 compatible solution of tenuigenin and liensinine （30 mg·kg^-1） at 30 min before anesthesia， and model group and blank group were injected intraperitoneally with the same amount of 0.9% NaCl solution. The blood gas indicators， cortical electroencephalogram results， Morris water maze behavior and serum corticosterone content were compared among groups. Western blot was used to detect the expression levels of Bcl₂， Bax and Caspase?3 in hippocampus. The total duration， average duration and quantity of epileptic waves of cortical electroencephalogram in TCM group were lower than those in model group （P<0.05）. Results of positioning navigation experiment showed that the escape latency in TCM group was shorter than that in model group （P<0.05）. Results of spatial probe test showed that the exploration time in TCM group was longer than that in model group （P<0.05）. Serum corticosterone content of rats in model group was higher than that in blank group （P<0.05）， and the content in TCM group was lower than that in model group （P<0.05）. The expression level of Bcl₂/Bax in hippocampus of TCM group was higher than that of model group （P<0.05）， while the expression level of cleaved Caspase?3 was lower than that of model group （P<0.05）. Results showed that tenuigenin and liensinine can improve the sevofluraneinduced acute and longterm cognitive impairments in neonatal rats. The mechanism may be related to regulating the expressions of apoptosisrelated genes Bcl₂， Bax and Caspase?3.

Key words: sevoflurane, tenuigenin, liensinine, Bcl₂, Bax, Caspase?3

中图分类号:

R614

韩丽丽, 王惠霞, 兰新昂, 韩慧梅, 孙洪超. 远志皂苷与莲心碱对七氟烷诱发新生大鼠急性和远期认知功能损伤的改善作用及机制分析[J]. 生物技术进展, 2021, 11(6): 807-812.

Lili HAN, Huixia WANG, Xinang LAN, Huimei HAN, Hongchao SUN. Analysis of Improvement Effect and Mechanism of Tenuigenin and Liensinine on Sevoflurane‑induced Acute and Longterm Cognitive Impairments of Neonatal Rats[J]. Current Biotechnology, 2021, 11(6): 807-812.

图/表 6

表1 各组新生SD大鼠血气指标比较（xˉ±s，n=12）

Table 1 Comparison of blood gas indicators of neonatal SD rats in each group (xˉ±s, n=12)

组别	PaO₂/mmHg	PaCO₂/mmHg	pH
空白组	105.91±3.94	42.12±1.73	7.41±0.06
模型组	103.47±4.12	43.85±1.97	7.39±0.08
中药组	104.13±4.26	43.61±2.38	7.37±0.05
F	1.394	2.522	1.152
P	0.262	0.096	0.328

表2 各组新生SD大鼠皮质脑电图结果比较（xˉ±s，n=12）

Table 2 Comparison of cortical electroencephalogram results of neonatal SD rats in each group (xˉ±s, n=12)

组别	癫痫波总时长/s	癫痫波平均时长/s	癫痫波数量/个
空白组	0.00±0.00	0.00±0.00	0.00±0.00
模型组	75.62±13.19^*	6.13±1.20^*	5.57±1.32^*
中药组	38.17±6.04^*#	3.34±0.51^*#	2.08±0.59^*#
F	244.548	199.459	136.421
P	<0.001	<0.001	<0.001

表3 各组新生SD大鼠定位航行实验结果比较

Table 3 （xˉ±s，n=12）Comparison of results of positioning navigation experiment of neonatal SD rats in each group (xˉ±s, n=12)

组别	游泳速度/（cm·s^-1）	逃避潜伏期/s
空白组	22.16±3.28	35.67±7.03
模型组	21.59±3.13	55.24±8.69^*
中药组	20.47±3.45	40.99±7.26^*#
F	0.820	20.750
P	0.449	<0.001

表4 各组新生SD大鼠空间探索实验结果比较

Table 4 （xˉ±s，n=12）Comparison of results of spatial probe test of neonatal SD rats in each group (xˉ±s, n=12)

组别	探索时间/s	穿越平台次数/次	目标象限停留时间/s
空白组	71.03±11.85	2.10±0.59	19.86±4.43
模型组	53.26±9.41^*	2.47±0.63	23.13±5.04
中药组	68.47±10.36^*#	2.25±0.61	21.59±4.80
F	9.878	1.116	1.415
P	<0.001	0.340	0.257

表5 各组新生SD大鼠血清皮质酮含量比较（xˉ±s，n=12）

Table 5 Comparison of serum corticosterone content of neonatal SD rats in each group (xˉ±s, n=12)

组别	皮质酮/（ng·mL）
空白组	82.59±27.18
模型组	463.80±62.51^*
中药组	137.21±48.94^*#
F	217.258
P	<0.001

图1 各组新生SD大鼠海马Bcl2、Bax和cleaved Caspase?3表达水平比较A：Western Blot图；B：各组大鼠海马Bcl2 /Bax和cleaved Caspase?3表达水平。*表示与空白组相比差异具有统计学意义（P<0.05）；#表示与模型组相比差异具有统计学意义（P<0.05）。

Fig. 1 Comparison of expression levels of Bcl2, Bax and cleaved Caspase?3 in the hippocampus of neonatal SD rats in each group

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远志皂苷与莲心碱对七氟烷诱发新生大鼠急性和远期认知功能损伤的改善作用及机制分析

Analysis of Improvement Effect and Mechanism of Tenuigenin and Liensinine on Sevoflurane‑induced Acute and Longterm Cognitive Impairments of Neonatal Rats

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