关键词: 转基因玉米, G1105E-823C转化体, 定性检测, 普通PCR, 实时荧光PCR

Abstract: Transgenic herbicide-resistant maize G1105E-823C is a new glyphosate-resistant transgenic maize line with modified mG2-aroA gene. It has a higher tolerance to glyphosate-herbicide. At present, this strain has completed production test and has important industrial application prospect in China. However, there is no relevant report on the specific detection method of transformants for G1105E-823C, which is very unfavorable for the detection and supervision of this strain. Based on this, the ordinary PCR and real-time fluorescence PCR qualitative detection methods of transgenic herbicide-resistant maize G1105E-823C were established with the specific sequence of G1105E-823C transformant as the target. The results showed that both methods could detect the components of transgenic herbicide-resistant maize G1105E-823C transformants with high specificity. The detection limit of ordinary PCR detection method was 0.1%, and that of real-time fluorescence PCR detection method was 0.05%. The two qualitative detection methods established in this study provided new technical means for the accurate detection of transgenic herbicide-resistant maize G1105E-823C, and provided technical support for agricultural transgenic supervision.

Key words: genetically modified maize, G1105E-823C event, qualitative detection, ordinary PCR, real-time fluorescence PCR