关键词: 玻璃粉, 纯化柱, 吸附, 核酸

Abstract: In order to study the application of nucleic acid extraction from plant tissue, the optimum condition of glass powder binding to nucleic acid was gained by comparing the effects of diameter of glass powder, chaotropic salt kinds and concentrations, pH on nucleic acid binding to glass powder. The results showed that glass powder had higher ability to absorb nucleic acid than silica and diatomite, and glass powder with 83 μm diameter was better. The glass powder has the strongest absorption ability under pH 4.0. The NaCl concentration should be higher then 3 mol/L in order to gain the highest yield of DNA, and glass powder can better absorb RNA with more than 2 mol/L guanidine thiocyanate. Besides, it was more effective by adding more than 50% anhydrous ethanol before glass powder absorb RNA. Nucleic acid extracted from plant tissue through the simple purification column, that was made by glass powder, can be applied for restriction digestion, hybridization and PCR. Compared with traditional methods, using glass powder column to entract nucleic acid from plant tissue is effective, envionmentally friendly, fast and economic.