关键词: PCR增强剂, 核酸检测, 纳米材料

Abstract: Under the background of the widespread prevalence of diseases caused by various highly pathogenic pathogens, avian influenza viruses, food-borne microorganisms, etc., it is particularly important to use PCR technology for rapid laboratory diagnosis of the first or first wave of cases. At the same time, a variety of PCR-based detection technologies have been developed to diagnose, prevent or predict diseases more quickly, with high throughput and sensitivity. However, in the detection of actual pathogens, the results of low sensitivity and poor accuracy often occur, so the PCR enhancer was added in the PCR amplification system. The PCR enhancer could improves the performance of PCR in terms of yield, specificity, sensitivity, etc. so as to optimize nucleic acid detection, solve the application bottleneck of pathogen detection, and save valuable time for the first pathogen detection. This review introduced the application and mechanism of PCR enhancer in combination with PCR-based nucleic acid in vitro amplification detection technology, in order to provide some reference for the practical application of nucleic acid detection.

Key words: PCR enhancer, nucleic acid detection, nanophase materials