功能核酸概念的内涵与外延

doi:10.19586/j.2095-2341.2021.0051

生物技术进展 ›› 2021, Vol. 11 ›› Issue (4): 446-454.DOI: 10.19586/j.2095-2341.2021.0051

功能核酸概念的内涵与外延

许文涛(), 杨敏, 朱龙佼, 张洋子, 李宏宇, 杜再慧, 杨文平

中国农业大学营养与健康系，食品精准营养与质量控制教育部重点实验室，北京 100083

收稿日期:2021-04-10 接受日期:2021-06-22 出版日期:2021-07-25 发布日期:2021-08-02
作者简介:许文涛E-mail：xuwentao@cau.edu.cn
基金资助:
山东省重大科技创新工程项目(2019JZZY011014);北京市农业科技项目(PXM2021_014207_000037)

The Connotation and Extension of the Functional Nucleic Acid

Wentao XU(), Min YANG, Longjiao ZHU, Yangzi ZHANG, Hongyu LI, Zaihui DU, Wenping YANG

Key Laboratory of Precision Nutrition and Food Quality，Department of Nutrition and Health，China Agricultural University，Beijing 100083，China

Received:2021-04-10 Accepted:2021-06-22 Online:2021-07-25 Published:2021-08-02

摘要/Abstract

摘要：

对功能核酸概念的分析需要建立在对功能核酸研究的基础上，从内涵和外延两个方面来进行探析。从内涵来看，它是对具有特殊结构、执行特定生物功能的核酸分子的统称；从外延来看，它包括适体、核酸核酶、核糖开关、发光核酸、修饰核酸、功能核酸裁剪、核酸自组装、功能核酸纳米材料、核酸纳米酶、核酸药物、核酸补充剂以及DNA存储技术等。目前功能核酸已成功地应用于生物传感、生物成像、生物医学等诸多领域。对功能核酸这一概念进行了探讨，并尝试对其范畴、特点进行归纳总结，以期梳理和完善功能核酸的基本概念，促进该领域的进一步发展。

关键词: 核酸, 功能核酸, 内涵与外延

Abstract:

Functional nucleic acid can be understood from both connotation and extension based on the current research. From the connotation point of view， it covers nucleic acid molecules with special structural functions and performing specific biological functions； from the perspective of extension， it includes aptamers， nucleic acid ribozymes， riboswitches， luminescent nucleic acids， modified nucleic acids， nucleic acid tailoring， nucleic acid self?assembly， nucleic acid nanomaterials， nucleic acid nanozymes， nucleic acid drugs， and nucleic acid supplements. At present， functional nucleic acids have been successfully applied in several fields， such as biosensing， bioimaging， and biomedicine. This article discussed the concept of functional nucleic acid， and attempted to summarize its category and characteristics， in order to sort out the basic concepts of functional nucleic acid and promote the further development of this field.

Key words: nucleic acid, functional nucleic acid, connotation and extension

中图分类号:

Q527

许文涛, 杨敏, 朱龙佼, 张洋子, 李宏宇, 杜再慧, 杨文平. 功能核酸概念的内涵与外延[J]. 生物技术进展, 2021, 11(4): 446-454.

Wentao XU, Min YANG, Longjiao ZHU, Yangzi ZHANG, Hongyu LI, Zaihui DU, Wenping YANG. The Connotation and Extension of the Functional Nucleic Acid[J]. Current Biotechnology, 2021, 11(4): 446-454.

图/表 2

参考文献 86

1	ELLINGTON A D, SZOSTAK J W. In vitro selection of RNA molecules that bind specific ligands [J]. Nature, 1990, 346(6287): 818-822.
2	TUERK C, GOLD L. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase [J]. Science, 1990, 249(4968): 505-510.
3	DARMOSTUK M, RIMPELOVA S, GBELCOVA H, et al.. Current approaches in SELEX: An update to aptamer selection technology [J]. Biotechnol. Adv., 2015, 33(6): 1141-1161.
4	ABABNEH N, ALSHAER W, ALLOZI O, et al.. In vitro selection of modified RNA aptamers against CD44 cancer stem cell marker [J]. Nucl. Acid Ther., 2013, 23(6): 401-407.
5	BOSHTAM M, ASGARY S, KOUHPAYEH S, et al.. Aptamers against pro- and anti-inflammatory cytokines: a review [J]. Inflammation, 2017, 40(1): 340-349.
6	YERRAMILLI V S, KIM K H. Labeling RNAs in live cells using malachite green aptamer scaffolds as fluorescent probes [J]. ACS Synth. Biol., 2018, 7(3): 758-766.
7	WANG J, WANG Y, HU X, et al.. Dual-aptamer-conjugated molecular modulator for detecting bioactive metal ions and inhibiting metal-mediated protein aggregation [J]. Anal. Chem., 2019, 91(1): 823-829.
8	YAN A C, LEVY M. Aptamer-mediated delivery and cell-targeting aptamers: room for improvement [J]. Nucl. Acid Ther., 2018, 28(3): 194-199.
9	ABDELRASOUL G N, ANWAR A, MACKAY S, et al.. DNA aptamer-based non-faradaic impedance biosensor for detecting E. coli [J]. Anal. Chim. Acta, 2020, 1107: 135-144.
10	ZHANG Y, LAI B S, JUHAS M. Recent advances in aptamer discovery and applications [J]. Molecules, 2019, 24(5): 941-962.
11	ACHENBACH J, CHIUMAN W, CRUZ R, et al.. DNAzymes: from creation in vitro to application in vivo [J]. Curr. Pharm. Biotechnol., 2004, 5(4): 321-336.
12	ZAOURI N, CUI Z, PEINETTI A S, et al.. DNAzyme-based biosensor as a rapid and accurate verification tool to complement simultaneous enzyme-based media for E. coli detection [J]. Environ. Sci. Water Res. Technol., 2019, 5(12): 2260-2268.
13	ROTHENBROKER M, MCCONNELL E M, GU J, et al.. Selection and characterization of an RNA‐cleaving DNAzyme activated by legionella pneumophila [J]. Angew. Chem., 2021, 133(9): 4832-4838.
14	ZHANG S, LUAN Y, XIONG M, et al.. DNAzyme amplified aptasensing platform for ochratoxin A detection using a personal glucose meter [J]. ACS Appl. Mater. Interf., 2021, 13(8): 9472-9481.
15	NAHVI A, SUDARSAN N, EBERT M S, et al.. Genetic control by a metabolite binding mRNA [J]. Chem. Biol., 2002, 9(9): 1043-1049.
16	沃森J D, 贝克T A,贝尔S P. 基因的分子生物学 [M]. 杨焕明译. 北京:科学出版社,2009.
17	冯婧娴, 王佳稳, 林俊生, 等. 核酶核糖开关在基因治疗中的应用及挑战 [J]. 药学学报, 2014, 11: 1504-1511.
18	毕茹茹, 范文廷, 马萍, 等. 核糖开关在细菌耐药性中的应用进展 [J]. 临床与病理杂志, 2016, 09(9): 1445-1449.
19	BEISEL C L, SMOLKE C D, ARKIN A P. Design principles for riboswitch function [J/OL]. PLoS Comp. Biol., 2009, 5(4): e1000363[2021-07-09]. .
20	刘湛军, 路新枝, 姜鹏, 等. 核糖开关——药物开发的新靶点 [J]. 武汉大学学报(理学版), 2010, 56(3): 313-319.
21	韩祥东, 刘薇, 吴存祥, 等. 核糖开关与基因表达调控 [J]. 中国生物化学与分子生物学报, 2011, 27(12): 1094-1100.
22	WANG X X, ZHU L J, LI S T, et al.. Fluorescent functional nucleic acid: principles, properties and applications in bioanalyzing [J]. Trac. Trend Anal. Chem., 2021, 116292-116325.
23	ZHOU Z, DING Y, SI S, et al.. Wide-field determination of aqueous mercury(II) based on tail-extensible DNA fluorescent probe with tunable dynamic range [J]. J. Hazard. Mater., 2021, 417:125975-125982.
24	TAN X, CONSTANTIN T P, SLOANE K L, et al.. Fluoromodules consisting of a promiscuous RNA aptamer and red or blue fluorogenic cyanine dyes: selection, characterization, and bioimaging [J]. J. Am. Chem. Soc., 2017, 139(26): 9001-9009.
25	DAVID P, CARDWELL L N, TAWIAH K D, et al.. Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines [J]. Nat. Commun., 2018, 9(1): 2283-2295.
26	LORENZ C, HADWIGER P, JOHN M, et al.. Steroid and lipid conjugates of siRNAs to enhance cellular uptake and gene silencing in liver cells [J]. Bioorg. Med. Chem. Lett., 2004, 14(19): 4975-4977.
27	NAIR J K, ATTARWALA H, SEHGAL A, et al.. Impact of enhanced metabolic stability on pharmacokinetics and pharmacodynamics of GalNAc-siRNA conjugates [J]. Nucl. Acids Res., 2017, 45(19): 10969-10977.
28	WAN W B, SETH P P. The medicinal chemistry of therapeutic oligonucleotides [J]. J. Med. Chem., 2016, 59(21): 9645-9667.
29	CHEN Z, LIU C, CAO F, et al.. DNA metallization: principles, methods, structures, and applications [J]. Chem. Soc. Rev., 2018, 47(11): 4017-4072.
30	YANG C, POHL R, TICHY M, et al.. Synthesis, photophysical properties, and biological profiling of benzothieno-fused 7-deazapurine ribonucleosides [J]. J. Org. Chem., 2020, 85(12): 8085-8101.
31	BING T, YANG X J, MEI H C, et al.. Conservative secondary structure motif of streptavidin-binding aptamers generated by different laboratories [J]. Bioorgan. Med. Chem., 2010, 18(5): 1798-1805.
32	PADLAN C S, MALASHKEVICH V N, ALMO S C, et al.. An RNA aptamer possessing a novel monovalent cation-mediated fold inhibits lysozyme catalysis by inhibiting the binding of long natural substrates [J]. RNA, 2014, 20(4): 447-461.
33	XIAO X N, ZHU L J, HE W C, et al.. Functional nucleic acids tailoring and its application [J]. Trac. Trend Anal. Chem., 2019, 118: 138-157.
34	LI M X, XU C H, ZHANG N, et al.. Exploration of the kinetics of toehold-mediated strand displacement via plasmon rulers [J]. ACS Nano, 2018, 12(4): 3341-3350.
35	WU C, CANSIZ S, ZHANG L, et al.. A nonenzymatic hairpin DNA cascade reaction provides high signal gain of mRNA imaging inside live cells [J]. J. Am. Chem. Soc., 2015, 137(15): 4900-4903.
36	YIN P, CHOI H M, CALVERT C R, et al.. Programming biomolecular self-assembly pathways [J]. Nature, 2008, 451(7176): 318-322.
37	RINKER S, KE Y, LIU Y, et al.. Self-assembled DNA nanostructures for distance-dependent multivalent ligand-protein binding [J]. Nat. Nanotechnol., 2008, 3(7): 418-422.
38	SEEMAN N C. Nucleic acid junctions and lattices [J]. J. Theor. Biol., 1982, 99(2): 237-247.
39	SEEMAN N C. DNA Nanotechnology: from the pub to information-based chemistry [J]. Methods Mol. Biol., 2018, 1811:1-9.
40	HU Y, NIEMEYER C M. From DNA nanotechnology to material systems engineering [J]. Adv. Mater., 2019, 31(26): e1806294[2021-07-09]. .
41	VYBORNYI M, VYBORNA Y, HANER R. DNA-inspired oligomers: from oligophosphates to functional materials [J]. Chem. Soc. Rev., 2019, 48(16): 4347-4360.
42	PINHEIRO A V, HAN D R, SHIH W M, et al.. Challenges and opportunities for structural DNA nanotechnology [J]. Nat. Nanotechnol., 2011, 6(12): 763-772.
43	KIM J, JANG D, PARK H, et al.. Functional-DNA-driven dynamic nanoconstructs for biomolecule capture and drug delivery [J]. Adv. Mater., 2018, 30(45): e1707351[2021-07-09]. .
44	XU X, WANG L, LI K, et al.. A smart DNA tweezer for detection of human telomerase activity [J]. Anal. Chem., 2018, 90(5): 3521-3530.
45	YURKE B, TURBERFIELD A J, MILLS A P, et al.. A DNA-fuelled molecular machine made of DNA [J]. Nature, 2000, 406(6796): 605-608.
46	ZHANG J, WANG L L, HOU M F, et al.. A ratiometric electrochemical biosensor for the exosomal microRNAs detection based on bipedal DNA walkers propelled by locked nucleic acid modified toehold mediate strand displacement reaction [J]. Biosens. Bioelect., 2018, 102: 33-40.
47	JUNG C, ALLEN P B, ELLINGTON A D. A simple, cleated DNA walker that hangs on to surfaces [J]. ACS Nano, 2017, 11(8): 8047-8054.
48	XUAN F, FAN T W, HSING I M. Electrochemical interrogation of kinetically-controlled dendritic DNA/PNA assembly for immobilization-free and enzyme-free nucleic acids sensing [J]. ACS Nano, 2015, 9(5): 5027-5033.
49	ANG Y S, YUNG L Y. Engineering self-contained DNA circuit for proximity recognition and localized signal amplification of target biomolecules [J]. Nucl. Acids Res., 2014, 42(14): 9523-9530.
50	XU W, HE W, DU Z, et al.. Functional nucleic acids‐nanomaterials: development, properties, and applications [J]. Angew. Chem. Intern. Edit., 2019, 60(13): 6890-6918.
51	马翾, 张洋子, 许文涛. 功能核酸DNA水凝胶的制备与组装 [J]. 生物技术进展, 2019, 9(6): 554-562.
52	马翾, 张洋子, 许文涛. 功能核酸DNA水凝胶的理化特性及应用进展 [J]. 生物技术进展, 2019, 9(6): 545-553.
53	ZHANG Y, ZHU L, TIAN J, et al.. Smart and functionalized development of nucleic acid-based hydrogels: assembly strategies, recent advances and challenges [J]. Adv. Sci., 2021, 2100216: 1-28.
54	LIU J. DNA-stabilized, fluorescent, metal nanoclusters for biosensor development [J]. Trends Anal. Chem., 2014, 58: 99-111.
55	XU J, ZHU X, ZHOU X, et al.. Recent advances in the bioanalytical and biomedical applications of DNA-templated silver nanoclusters [J]. Trends Anal. Chem., 2020, 124: 115786-115800.
56	SATYAVOLU N S R, LOH K Y, TAN L H, et al.. Discovery of and Insights into DNA "Codes" for Tunable Morphologies of Metal Nanoparticles [J]. Small, 2019, 15(26): e1900975[2021-07-12]..
57	O'NEILL P R, YOUNG K, SCHIFFELS D, et al.. Few-atom fluorescent silver clusters assemble at programmed sites on DNA nanotubes [J]. Nano Lett., 2012, 12(11): 5464-5469.
58	SU Y, CHU H, TIAN J, et al.. Insight into the nanomaterials enhancement mechanism of nucleic acid amplification reactions [J]. Trends Anal. Chem., 2021, 137: 116221-116237.
59	ZHOU W, FANG Y, REN J, et al.. DNA-templated silver and silver-based bimetallic clusters with remarkable and sequence-related catalytic activity toward 4-nitrophenol reduction [J]. Chem. Commun., 2019, 55(3): 373-376.
60	LU C, TANG L, GAO F, et al.. DNA-encoded bimetallic Au-Pt dumbbell nanozyme for high-performance detection and eradication of Escherichia coli O157:H7 [J]. Biosens. Bioelectr., 2021, 187: 113327-113336.
61	CHEN W, FANG X, YE X, et al.. Colorimetric DNA assay by exploiting the DNA-controlled peroxidase mimicking activity of mesoporous silica loaded with platinum nanoparticles [J]. Microchim. Acta, 2018, 185(12): 1-10.
62	A H M, WANG L, LI Y, et al.. Guanosine-rich aptamers Cu₂O nanoparticles: enhanced peroxidase activity and specific recognition capability at neutral pH [J]. Chem. Commun., 2021,57, 643-646.
63	MENG Y, CHEN Y, ZHU J, et al.. Polarity control of DNA adsorption enabling the surface functionalization of CuO nanozymes for targeted tumor therapy [J]. Mater. Horizons, 2021, 8(3): 972-986.
64	BREAKER R R, JOYCE G F. The expanding view of RNA and DNA function [J]. Chem. Biol., 2014, 21(9): 1059-1065.
65	ZOU Y, SUN X, WANG Y, et al.. Single siRNA nanocapsules for effective siRNA brain delivery and glioblastoma treatment [J]. Adv. Mater., 2020, 32(24): e2000416[2021-07-12]..
66	CHAKRABORTY C, SHARMA A R, SHARMA G, et al.. Therapeutic miRNA and siRNA: moving from bench to clinic as next generation medicine [J]. Mol. Ther. Nucl. Acids, 2017, 8: 132-143.
67	LI D, MASTAGLIA F L, FLETCHER S, et al.. Precision medicine through antisense oligonucleotide-mediated exon skipping [J]. Trends Pharmacol. Sci., 2018, 39(11): 982-994.
68	JAIN S, KAUR J, PRASAD S, et al.. Nucleic acid therapeutics: a focus on the development of aptamers [J]. Expert Opin. Drug Discov., 2021, 16(3): 255-274.
69	MAHJOUBIN-TEHRAN M, REZAEI S, ATKIN S L, et al.. Decoys as potential therapeutic tools for diabetes [J/OL]. Drug Discov. Today, 2021, doi:10.1016/j.drudis.2021.04.004[2021-07-09]. .
70	KHAN A U, LAL S K. Ribozymes: a modern tool in medicine [J]. J. Biomed. Sci., 2003, 10(5): 457-467.
71	REPP L, RASOULIANBOROUJENI M, LEE H J, et al.. Acyl and oligo(lactic acid) prodrugs for PEG-b-PLA and PEG-b-PCL nano-assemblies for injection [J]. Offic. J. Contr. Release Soc., 2021, 330: 1004-1015.
72	CHARLEBOIS R, ALLARD B, ALLARD D, et al.. PolyI:C and CpG synergize with anti-ErbB2 mAb for treatment of breast tumors resistant to immune checkpoint inhibitors [J]. Cancer Res., 2017, 77(2): 312-319.
73	WRAIGHT C J, WHITE P J. Antisense oligonucleotides in cutaneous therapy [J]. Pharmacol. Therap., 2001, 90(1): 89-104.
74	CAVAGNARI B M. Gene therapy: nucleic acids as drugs. Action mechanisms and delivery into the cell] [J]. Archiv. Argent. Pediat., 2011, 109(3): 237-244.
75	梁兴国, 李佥, 黄丽丽, 等. 核酸代谢与营养研究及发展趋势 [J]. 中国海洋大学学报(自然科学版), 2019, 10: 64-78.
76	KUBOTA A. Nutritional study of nucleotide components in the milk [J]. Nihon Shonika Gakkai Zasshi Acta Paed. Japon., 1969, 73(2):197-209.
77	EUN-HEE D, CHRISTOPHE C, SCHWAB C, et al.. Effect of dietary nucleosides and yeast extracts on composition and metabolic activity of infant gut microbiota in PolyFermS colonic fermentation models [J]. Fems Microbiol. Ecol., 2017,97(8):8[2021-07-12]..
78	XU M, LIANG R, GUO Q, et al.. Dietary nucleotides extend the life span in Sprague-Dawley rats[J]. J. Nutr. Health Aging, 2013, 17(3): 223-229.
79	OACUTE, PEZ-NAVARRO A T, GIL A, et al., Age-related effect of dietary nucleotides on liver nucleic acid content in rats [J]. Ann. Nutr. Metab., 1997, 41(5): 324-330.
80	CAI X, BAO L, WANG N, et al.. Dietary nucleotides protect against alcoholic liver injury by attenuating inflammation and regulating gut microbiota in rats [J]. Food Function, 2016, 7(6): 2898-2908.
81	CEZE L, NIVALA J, STRAUSS K. Molecular digital data storage using DNA[J]. Nat. Rev. Genet., 2019, 20: 456-466.
82	ORGANICK L, ANG S D, CHEN Y J, et al.. Random access in large-scale DNA data storage [J]. Nat. Biotechnol., 2018, 36(3): 242-250.
83	HECKEL R, MIKUTIS G, GRASS R N. A characterization of the DNA data storage channel [J]. Sci. Rep., 2019, 9(1): 1-12.
84	FARZADFARD F. DNA storage in everyday objects [J]. Nat. Biotechnol.,2020,38: 31-32.
85	LIM C K, NIRANTAR S, YEW W S,et al.. Novel modalities in DNA data storage[J/OL]. Trends Biotechnol., 2021,doi:10.1016/j.tibtech.2020.12.008[2021-07-09]. .
86	BANAL J L, SHEPHERD T R, BERLEANT J, et al.. Random access DNA memory using Boolean search in an archival file storage system[J/OL]. Nat. Mater, 2021,doi: 10.1038/s41563-021-01021-3[2021-07-09]. .

类型	靶物质	作用位点	作用机制	总结	参考文献
siRNA	mRNA	细胞内(细胞质)	mRNA切割	根据RNAi的原理，具有与序列（siRNA）同源的mRNA切割的双链RNA，单链发夹RNA（shRNA）等	[65]
miRNA	microRNA	细胞内(细胞质)	microRNA替代	双链RNA，单链发夹RNA的miRNA或其模拟物可用于增强因疾病而恶化的miRNA的功能	[66]
反义核苷酸	mRNA、miRNA	细胞内(细胞核及细胞质)	mRNA和miRNA 降解及拼接抑制	单链RNA/DNA，与靶mRNA和miRNA结合，引起降解或抑制，或在剪接时跳过外显子	[67]
适体	蛋白质(胞外蛋白)	细胞内(细胞核及细胞质)	功能抑制	单链RNA/DNA，以与抗体/DNA相似的方式与靶蛋白结合	[68]
寡聚核苷酸	蛋白质(转录因子)	细胞内(细胞核)	转录抑制	与转录因子结合位点序列相同的双链DNA，其与受影响基因的转录因子结合以抑制靶基因	[69]
核酸核酶	RNA	细胞内(细胞质)	RNA切割	具有酶功能的单链RNA，可结合和裂解靶RNA	[70]
CpG oligo	蛋白质(受体)	细胞表面	免疫增强	具有CpG基序的寡脱氧核苷酸（单链DNA）	[71]
其他	—	—	—	除上面列出的核酸药物外，还可以激活先天免疫的核酸药物，例如PolyI：PolyC（双链RNA）和抗原	[72]

类型	靶物质	作用位点	作用机制	总结	参考文献
siRNA	mRNA	细胞内(细胞质)	mRNA切割	根据RNAi的原理，具有与序列（siRNA）同源的mRNA切割的双链RNA，单链发夹RNA（shRNA）等	[65]
miRNA	microRNA	细胞内(细胞质)	microRNA替代	双链RNA，单链发夹RNA的miRNA或其模拟物可用于增强因疾病而恶化的miRNA的功能	[66]
反义核苷酸	mRNA、miRNA	细胞内(细胞核及细胞质)	mRNA和miRNA 降解及拼接抑制	单链RNA/DNA，与靶mRNA和miRNA结合，引起降解或抑制，或在剪接时跳过外显子	[67]
适体	蛋白质(胞外蛋白)	细胞内(细胞核及细胞质)	功能抑制	单链RNA/DNA，以与抗体/DNA相似的方式与靶蛋白结合	[68]
寡聚核苷酸	蛋白质(转录因子)	细胞内(细胞核)	转录抑制	与转录因子结合位点序列相同的双链DNA，其与受影响基因的转录因子结合以抑制靶基因	[69]
核酸核酶	RNA	细胞内(细胞质)	RNA切割	具有酶功能的单链RNA，可结合和裂解靶RNA	[70]
CpG oligo	蛋白质(受体)	细胞表面	免疫增强	具有CpG基序的寡脱氧核苷酸（单链DNA）	[71]
其他	—	—	—	除上面列出的核酸药物外，还可以激活先天免疫的核酸药物，例如PolyI：PolyC（双链RNA）和抗原	[72]

[1]	赵文卓, 李成勋, 胡作建, 余红秀. 功能核酸用于致病菌检测的研究进展[J]. 生物技术进展, 2023, 13(1): 30-38.
[2]	陈可仁, 汪未申, 朱龙佼, 张洋子, 贺晓云, 黄昆仑, 许文涛. 核酸自组装纳米递送载体的研究进展[J]. 生物技术进展, 2022, 12(3): 352-357.
[3]	格日乐其木格, 牛振峰, 董丹, 张涛涛, 峥嵘. CRISPR-Cas系统在微生物研究中的应用进展[J]. 生物技术进展, 2021, 11(3): 253-259.
[4]	刘旺, 靳晶豪, 陈孝仁. 环介导等温扩增技术的应用进展[J]. 生物技术进展, 2021, 11(2): 128-135.
[5]	杨佳怡,陈桂芳,高运华,王志栋,吴枭. HPV核酸检测标准物质研究进展[J]. 生物技术进展, 2020, 10(6): 590-596.
[6]	胡思宏,游国叶. 数字PCR在新型冠状病毒检测中的应用前景[J]. 生物技术进展, 2020, 10(6): 674-679.
[7]	任雯,杨海侠,陈立柱,李雨峰,刘亚. 核酸层析法转基因快速检测体系的建立及应用[J]. 生物技术进展, 2020, 10(6): 680-687.
[8]	徐蕾，肖桂清，盛晓菁，戚智青，刁勇. PCR增强剂在核酸体外扩增检测技术的研究进展[J]. 生物技术进展, 2020, 10(2): 137-143.
[9]	马翾,张洋子,许文涛,. 功能核酸DNA水凝胶的理化特性及应用进展[J]. 生物技术进展, 2019, 9(6): 545-553.
[10]	马翾,张洋子,许文涛,. 功能核酸DNA水凝胶的制备与组装[J]. 生物技术进展, 2019, 9(6): 554-562.
[11]	杨文平,,吴远根. 基于Fe₃O₄过氧化物酶活性的Cd²⁺和Pb²⁺比色传感器[J]. 生物技术进展, 2019, 9(6): 611-619.
[12]	粟元,李舒婷,许文涛,. 气体生物传感器的应用研究进展[J]. 生物技术进展, 2019, 9(6): 627-632.
[13]	黄义,张维,燕永亮. 细菌RNA稳定性调控相关元件的研究进展[J]. 生物技术进展, 2018, 8(5): 376-382.
[14]	彭志,陈刚毅,刘雪飞,黄新河. 等温核酸扩增技术在病原体检测中的应用[J]. 生物技术进展, 2018, 8(4): 284-292.
[15]	何海旺,许春燕,李文,何龙飞,李创珍,韦善清. 玻璃粉吸附核酸及其在植物核酸提取中应用的研究[J]. 生物技术进展, 2012, 2(5): 359-365.

功能核酸概念的内涵与外延

The Connotation and Extension of the Functional Nucleic Acid

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 86

相关文章 15

编辑推荐

Metrics

本文评价