关键词: 叶酸, AtDFB基因, 低氮胁迫, 发育, 拟南芥

Abstract: Folylpolyglutamate synthetase (FPGS) catalyzes the addition of glutamate residues to the γ-carboxyl of tetrahydrofolate in different positions, to form folylpolyglutamates. AtDFB is the FPGS that located in plastid of Arabidopsis thaliana. In this study the Arabidopsis T-DNA insertion mutant (Atdfb-3) of AtDFB gene was used to explore the function of AtDFB gene by comparing the phenotype of wild type and atdfb-3 under various nitrogen conditions. The results showed that Atdfb-3 mutant displayed shorter primary root length and decreased folate content, compared with the wild type under the nitrogen-limited condition. The phenotype of AtDFB overexpression line under Atdfb-3 background restored to the wild-type level, and the folate content increased under the nitrogen-limited condition. In conclusion, Arabidopsis AtDFB gene plays an important role in folate biosynthesis pathway, and affects the primary root development under the nitrogen-limited condition.

Key words: folate, AtDFB gene, nitrogen-limited condition, development, Arabidopsis