关键词: 拟南芥, AtEXD, 酵母双杂交, 盐胁迫

Abstract: Various stresses have bad effects on plants in the process of plant growth and development. Among them, salt stress has always been one of the abiotic stresses that have the worst impact on plant growth and crop yield. When studying Arabidopsis salt stress, we discovered gene AtEXD (At2g25910) responding to salt stress. By the analysis of its basic characteristics, we learned that AtEXD had a 3′-5′ exonuclease domain and a KH domain and were highly conservative in evolutionary relationships. In contrast, we know very little about its function now. By the yeast two-hybrid, we screened some transcription factors interacting with AtEXD and the results of functional analysis showed that AtEXD might be involved in the stress response mechanism of plants. The statistical results of seeds germination rate showed that the mutants exd-1 and exd-2 had salt-sensitive phenotypes. When AtEXD was overexpressed, the salt tolerance of Arabidopsis was significantly enhanced. These results indicated that AtEXD probably regulate the salt tolerance of plants.

Key words: Arabidopsia thaliana, AtEXD, yeast two-hybrid, salt stress