右美托咪定对慢性应激抑郁妊娠大鼠子代发育及空间学习记忆能力的影响

doi:10.19586/j.2095-2341.2021.0032

生物技术进展 ›› 2022, Vol. 12 ›› Issue (1): 135-141.DOI: 10.19586/j.2095-2341.2021.0032

右美托咪定对慢性应激抑郁妊娠大鼠子代发育及空间学习记忆能力的影响

王向阳¹(), 刘安军¹, 赵晶²

^1.中国人民解放军联勤保障部队第960医院麻醉科，济南 250031
^2.中国人民解放军联勤保障部队第960医院康复医学科，济南 250031

收稿日期:2021-03-09 接受日期:2021-06-16 出版日期:2022-01-25 发布日期:2022-01-26
通讯作者: 赵晶
作者简介:王向阳 E-mail：mirgl78@126.com；

Effects of Dexmedetomidine on Offspring Development, Spatial Learning and Memory Ability of Pregnancy Rats with Chronic Stress Depression

Xiangyang WANG¹(), Anjun LIU¹, Jing ZHAO²

^1.Department of Anesthesiology，the 960th Hospital of the PLA Joint Logistics Suppor Force，Jinan 250031，China
^2.Department of Rehabilitation Medicine，the 960th Hospital of the PLA Joint Logistics Suppor Force，Jinan 250031，China

Received:2021-03-09 Accepted:2021-06-16 Online:2022-01-25 Published:2022-01-26
Contact: Jing ZHAO

摘要/Abstract

摘要：

妊娠期抑郁症可以通过影响孕妇的生理和心理变化，进而影响新生儿的正常生长发育，右美托咪定（dexmedetomidine，Dex）可以有效缓解抑郁情绪，降低产后抑郁症的发生。为探讨Dex对慢性应激抑郁妊娠大鼠子代发育及空间学习记忆能力的影响，将60只妊娠大鼠随机分为对照组、模型组、低剂量右美托咪定组和高剂量右美托咪定组，统计各组大鼠孕育率、平均产仔率和平均仔鼠成活率，并记录子代大鼠出生后体质量变化及学习记忆能力；检测子鼠脑组织乙酰胆碱（acetylcholine，Ach）、5?HT含量、TChE活力及海马组织CREB、p?CREB及BDNF的表达。结果显示，Dex可以以剂量依赖的方式提高慢性应激抑郁妊娠大鼠子代的平均成活率，调节脑组织Ach、5?HT、CREB、p?CREB和BDNF的水平，改善子鼠的学习记忆能力。结果表明，Dex可能通过调控神经递质和促进CREB信号传导，改善慢性应激抑郁妊娠大鼠的子代鼠学习记忆能力。研究探究了Dex对妊娠期抑郁症大鼠子代生长发育和空间学习记忆能力的影响，以期指导临床治疗。

关键词: 妊娠大鼠, 子鼠发育, 学习记忆能力, 右美托咪定

Abstract:

Pregnancy depression can affect the normal growth and development of newborns by affecting the physiological and psychological changes of pregnant women. Dexmedetomidine （Dex） can effectively relieve the depression and reduce the occurrence of postpartum depression. To explore the effects of Dex on offspring development， spatial learning and memory ability of pregnancy rats with chronic stress depression， sixty female pregnant rats were randomly divided into control group， model group， low?dose Dex group and high?dose Dex group. The inoculation rate， average farrowing rate and average survival rate of offspring rats in each group were statistically analyzed. The body mass change and learning and memory ability of offspring rats were recorded after birth. The levels of acetylcholine （Ach）， 5?HT and TChE in brain tissues， and expression of CREB， p?CREB and BDNF in hippocampus of offspring rats were detected. The study showed that Dex could increase the average survival rate by a dose?dependent manner， promoted the levels of Ach， 5?HT， CREB， p?CREB and BDNF levels in the brain tissue， and improved the learning and memory ability of the offspring of chronic stress depression pregnant rats. The results showed that Dex may improve the learning and memory ability of offspring of pregnant rats with chronic stress depression by regulating neurotransmitters and promoting CREB signal transduction. This study aims to explore the effects of Dex on the growth and development and spatial learning and memory abilities of the offspring of rats with depression during pregnancy， so as to guide clinical treatment.

Key words: pregnancy rat, offspring mouse development, learning and memory ability, dexmedetomidine

中图分类号:

R?332

王向阳, 刘安军, 赵晶. 右美托咪定对慢性应激抑郁妊娠大鼠子代发育及空间学习记忆能力的影响[J]. 生物技术进展, 2022, 12(1): 135-141.

Xiangyang WANG, Anjun LIU, Jing ZHAO. Effects of Dexmedetomidine on Offspring Development, Spatial Learning and Memory Ability of Pregnancy Rats with Chronic Stress Depression[J]. Current Biotechnology, 2022, 12(1): 135-141.

图/表 6

表1 4组大鼠中央区停留时间和糖水消耗比较（x±s）

Table 1 Comparison of residence time and sugar water consumption in central area of rats among four groups (x±s)

组别（n=15）	中央区停留时间/s	糖水消耗/mL
组别（n=15）	中央区停留时间/s	0 d	18 d
对照组	4.87±0.73	0.85±0.02	0.84±0.02
模型组	11.23±2.14^*	0.86±0.03	0.50±0.02^*
低剂量右美托咪定组	8.68±1.73^*^#	0.86±0.04	0.71±0.04^*^#
高剂量右美托咪定组	7.32±1.56^#&	0.86±0.04	0.77±0.03^#&
F值	45.354	0.562	26.178
P值	<0.001	0.631	<0.001

表2 4组大鼠孕育率、平均产仔率和平均仔鼠成活率比较（x±s）

Table 2 Comparison of inoculation rate, average farrowing rate and average survival rate among four groups (x±s)

组别（n=15）	孕育率/%	子代数/只	子代平均成活数/只	子代成活率/%
对照组	100.00	10.80±1.15	10.00±1.21	92.59±3.21
模型组	100.00	11.53±1.02	7.66±1.09^*	72.78±2.32^*
低剂量右美托咪	100.00	10.67±1.08	9.33±0.97^*#	87.50±2.36^*#
高剂量右美托咪定组	100.00	10.86±1.45	10.07±1.34^#&	92.64±2.11^#&
F值	0.000	1.579	13.987	18.421
P值	1.000	0.205	<0.001	<0.001

图1 4组子鼠出生后体质量比较

Fig. 1 Comparison of weight of offspring rats after birth among four groups

图2 4组子鼠的学习记忆能力检测结果比较注：*表示与对照组相比，差异具有统计学意义（P<0.05）；#表示与模型组相比，差异具有统计学意义（P<0.05）；&表示与低剂量右美托咪定组相比，差异具有统计学意义（P<0.05）。

Fig.2 Test results comparison of learning and memory abilities of offspring rats among four groups

图3 4组子鼠脑组织5?HT、Ach和TChE含量水平比较注：*表示与对照组相比，差异具有统计学意义（P<0.05）；#表示与模型组相比，差异具有统计学意义（P<0.05）；&表示与低剂量右美托咪定组相比，差异具有统计学意义（P<0.05）。

Fig.3 Comparison of 5?HT, Ach and TChE levels in brain tissues among four groups

图4 4组大鼠脑组织CREB、p?CREB及BDNF蛋白含量水平比较注：A—对照组；B—模型组；C—低剂量右美托咪定组；D—高剂量右美托咪定组。*表示与对照组相比，差异具有统计学意义（P<0.05）；#表示与模型组相比，差异具有统计学意义（P<0.05）；&表示与低剂量右美托咪定组相比，差异具有统计学意义（P<0.05）。

Fig.4 Comparison of levels of CREB, p?CREB and BDNF proteins in brain tissues among four groups

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右美托咪定对慢性应激抑郁妊娠大鼠子代发育及空间学习记忆能力的影响

Effects of Dexmedetomidine on Offspring Development, Spatial Learning and Memory Ability of Pregnancy Rats with Chronic Stress Depression

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