关键词: 大鼠慢性束缚应激模型, 行为学评估, 肠道组织, 肠道菌群, 变化

Abstract: To establish the rat chronic stress model using the chronic stress method, and further observe the variation rule of rat intestinal pathology and its flora based on HE staining and ERIC-PCR technique. A total of 10 healthy male SD rats were chosen, and randomly divided into two groups: Control group and model group, 5 rats in each group. Wherein, rats in control group were regular raised, and rats in model group were perform constraint stress for 4 hours everyday using a constraint tube for 30 days, and the rats body weight was recorded before or after modeling, and then performed the behavioral evaluation. In addition, rats were killed, and the intestinal tissues and contents were collected, and then sliced to stain by HE staining, and the genomic DNA was extracted to perform ERIC-PCR assay. Results showed that, compared to control, the body weight, the number of through, upright, grooming  significantly decreased, the no moving time of forced swimming significantly prolonged, and sugar water preference significantly decreased. Furthermore, the microvilli of intestinal tract were seriously damaged, and nuclei mild contraction and deeply staining. The intestinal flora obviously changed, and several characteristic bands appeared. Rats chronic stress model was correctly established using chronic constraint stress methods and behavioral evaluation, and provided a significant reference on the study of the chronic stress associated diseases based on pathological assay and intestinal flora expression profile alteration, and exhibited a certain application value.

Key words: rats chronic constraint model, behavioral evaluation, intestinal tissue, intestinal flora, alteration