生物技术进展 ›› 2014, Vol. 4 ›› Issue (4): 236-244.DOI: 10.3969/j.issn.2095-2341.2014.04.02

• 进展评述 • 上一篇    下一篇

可视安全标记花青素合成途径相关基因在植物转化研究中的应用进展

杜建中,赵欣梅,王长彪,郝曜山,王亦学,张欢欢,孙毅*   

  1. 山西省农业科学院生物技术研究中心, 太原 030031
  • 收稿日期:2014-07-02 出版日期:2014-07-25 发布日期:2014-07-09
  • 通讯作者: 孙毅,研究员,博士生导师,主要从事植物分子生物学研究。E-mail:sunyi692003@163.com
  • 作者简介:杜建中,副研究员,硕士,主要从事植物基因工程研究。E-mail:dujianzhong1960@163.com。
  • 基金资助:

    国家自然科学基金(31240081);山西省攻关项目(20110311009)资助。

Advances on Visible and Bio-safe Selectable Marker Genes for Plant Transformation: the Genes for Anthocyanin Biosynthesis

DU Jian-zhong, ZHAO Xin-mei, WANG Chang-biao, HAO Yao-shan, WANG Yi-xue, ZHANG Huan-huan, SUN Yi   

  1. The Biotechnology Reseacher Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
  • Received:2014-07-02 Online:2014-07-25 Published:2014-07-09

摘要: 近年来有关转基因产品可能带来的环境和食品安全问题的争论多集中在作为标记基因的抗生素抗性基因以及抗除草剂基因的广泛使用。因此寻找安全、有效的标记基因替代上述有争议标记基因就显得十分必要和紧迫。花青素合成酶类及其合成调控因子可以控制植物体内的色素合成,一些转化研究已证明其转化体表型发生了颜色改变。加之花青素类物质是一些天然色素,对人类有利而无害,可以利用花青素的这些特点,将花青素合成酶类及其调控因子基因作为一类可视化、安全和有效的标记基因来转化植物。本文就可视安全标记花青素合成酶类基因及其调控基因在植物转化研究中的应用进展进行了简要介绍,以期为提高标记基因的安全性提供参考。

关键词: 可视基因, 花青素, 植物转化, 表型变化

Abstract: Controversy on environment and food safety of transgenic products mainly focused on its containing antibiotics and herbicides\|tolerant genes as selectable marker genes in recent years. Therefore, it is necessary and urgent to find safer and more efficient marker genes as alternatives. Some pigments, such as anthocyanin, synthesis in plants are controlled by anthocyanin synthesis enzymes and transcriptional regulatory factors. Some studies have demonstrated that visible color changes occurred in transformed phenotype by introducing these genes into plants. In addition, the products or intermediate products in anthocyanin biosynthesis pathway are all natural pigments which are benefitial to human health. Therefore, the genes or transcriptional regulatory factors responsible for anthocyanin biosynthesis could be used as selectable marker gene in plant transformation. In this paper, we gave a brief introduction of the research progress on plant transformation with visible marker genes, which was expected to provide a reference for selectable marker safety.

Key words: visible marker, anthocyanidin, plant transformation, phenotypic modulation