关键词: 无菌斑马鱼, 病毒感染模型, 鲤春病毒血症病毒, 肠道菌群

Abstract: Virus diseases cause serious damages to Chinese aquaculture industry, but there is no effective countermeasure yet. Recent studies have confirmed the involvement of intestinal microflora in the interaction between host and viruses. However, there are rare related studies on fish. Germ-free (GF) animal infection model is the prerequisite for investigation of intestinal microflora-related functions. Based on this, a stable and reliable GF zebrafish model for spring viraemia of carp virus (SVCV) infection was established. Further, this model was used to investigate the influence of intestinal microflora and 3 strains of representative commensal intestinal bacteria, including Cetobacterium somerae, Aeromonas veronii and Plesimonas shigelloides, on viral infection in zebrafish. The results showed that there was no significant influence of intestinal microflora or commensal bacteria on SVCV infection after bacteria colonizing the intestinal tract of fish over 24 h. The establishment of a GF zebrafish model for SVCV infection provided a new way for rapidly screening the bacterial strains which could affect virus infection. The study also provided a possibility for further identification of the antiviral effectors derived from commensal bacteria of fish.

Key words: germ-free zebrafish, virus infection model, spring viraemia of carp virus, intestinal microflora