关键词: 大鼠急性应激模型, HE染色, 行为学, 肠道菌群, ERIC-PCR

Abstract: In order to study the feasibility of establishing the acute stress model based on water-immersion and restraint stress, and observe the histopathological change of stomach, ileum, cecum and colon using HE staining, and further identify the change rule of intestinal flora profile by ERIC-PCR. The total  healthy male rats were randomly devided into two groups: control group and model group. Rats were regularly raised in control group, and  model group were placed in a homemade constraint cages after inhalation anesthesia with isoflurane, and then immersed in 23±2℃ water for 5h when it revived. Then, the rat body weight was recorded and had on open field test and forced swimming test. Finally, rats were dislocated to collect the tissues and its contents of stomach, ileum, cecum and colon to perform HE staining and ERIC-PCR identifying respectively. We gathered the images to study the gastrointestinal histopathological changes and its intestinal flora profile analysis using ERIC-PCR after acute stress. Comparing with control, the rat body weight had no significantly variation, the number of through significantly decreased, the number of upright significantly decreased, the number of grooming significantly decreased, and the no moving time of forced swimming significantly prolonged. Furthermore, the gastric mucosa and the microvilli of intestinal tract were not damaged, and no nuclei deeply staining, and several obviously changing characteristic bands in the intestinal flora were appeared. This study successfully established the acute stress model, and followed with HE staining and ERIC-PCR to provide a significant reference of studying on the intestinal flora profile based on rat acute stress model, and had a significant application value.

Key words: rat acute stress model, HE staining, behavior, intestinal flora, ERIC-PCR