生物技术进展 ›› 2014, Vol. 4 ›› Issue (1): 30-35.DOI: 10.3969/j.issn.2095-2341.2014.01.06

• 研究论文 • 上一篇    下一篇

一氧化氮处理对香蕉多聚半乳糖醛酸酶及MaPGs基因表达的影响

吴斌1,2，郭芹3,王刚霞2,陈维信1,李雪萍1*   

  1. 1.华南农业大学园艺学院， 广东省果蔬保鲜重点实验室， 广州 510642；
    2.新疆农业科学院农产品贮藏加工研究所， 乌鲁木齐 830091；
    3.中国农业科学院农产品加工研究所， 农产品贮藏保鲜与物流研究室， 北京 100193
  • 收稿日期:2013-10-14 出版日期:2014-01-25 发布日期:2013-11-08
  • 通讯作者: 李雪萍，副教授，博士，研究方向为采后保鲜与病理。E\|mail：lxp88@scau.edu.cn
  • 作者简介:吴斌，博士后，研究方向为果蔬贮藏与加工。E-mail: xjuwubin0320@sina.com
  • 基金资助:

    现代农业产业技术体系建设专项资金（CARS-32-09A）;广东省岭南水果产业技术体系项目(LNSG2011-2012)资助。

Effects of Nitric Oxide Treatment on Polygalacturonase Activity and Expression of MaPGs Genes in Harvested Banana Fruit During Storage

WU Bin, GUO Qin, WANG Gang-xia, CHEN Wei-xin, LI Xue-ping   

  1. 1.Guangdong Province Key Laboratory for Postharvest Technology of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, China;
    2.Institute of Agro\|products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;
    3.Preservation and Logistics Division, Institute of Agro\|products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2013-10-14 Online:2014-01-25 Published:2013-11-08

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摘要： 为了解多聚半乳糖醛酸酶（PG）在香蕉采后软化中的分子调控机制，采用40 μL/L NO熏蒸处理绿熟期的‘巴西’香蕉果实3 h后，在20℃和相对湿度为85%的条件下贮藏，研究NO对香蕉果实乙烯释放量、硬度、PG活性及MaPGs基因表达的影响。结果表明：NO处理降低了果实乙烯释放量，延缓了果实硬度的下降，抑制了PG的活性；降低了MaPG2、MaPG3和MaPG4基因的表达，延缓了香蕉果实的软化。

关键词: 一氧化氮, 香蕉, 软化, 多聚半乳糖醛酸酶, 基因

Abstract: In order to explore the molecular regulation mechanism of polygalacturonase (PG) during banana softening, fruit harvested at the mature green stage were fumigated with 40 μL/L of NO for 3 h, and then stored at 20℃ with 85% relative humility. Ethylene production, firmness, PG activity and MaPGs genes were measured during banana ripening. The results showed that NO treatment resulted in significantly lower rates of ethylene production than those in the control fruit. The NO treated fruit underwent a lesser loss of firmness during storage. The activity of PG and expression of MaPG2、MaPG3 and MaPG4 genes were effectively reduced in NO treated fruit, indicating that the softening of banana fruit was delayed by NO treatment.

Key words: nitric oxide, banana, softening, polygalacturonase, gene

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