关键词: 银柴胡, 离体培养, 发根农杆菌, 毛状根, 诱导

Abstract: In this study, stems of Stellaria dichotoma L. var. laceolata Bge as explants  were sterilized, and sterile culture material were acquired. Then its hairy roots were induced by Agrobacterium rhizogenes A4 with Ri-plasmid. Results showed the method  that the explants was soaked in 70% alcohol for 5 s and in 0.1% HgCl2 for 3 min, was the optimal sterilized method, so sterile culture material were acquired. During the induction by Agrobacterium rhizogenes A4, comparing with the leaves and stems with no axillary bub taking the stems with axillary bud was the best explant for transformation. The induction rate and the density was 100% and 4.7, respectively, when the stems with axillary bud were infected for 15 min and co-cultured for 3 days with Agrobacterium rhizogenes (OD600=0.8), and sterilized by 800 mg/L cefotaxime sodium. So these were the optimized factors, and the stems with axillary bud were the optimized explants for transformation.

Key words: Stellaria dichotoma L. var. laceolata Bge, isolated culture, Agrobacterium rhizogenes, hairy roots, induction