生物技术进展 ›› 2013, Vol. 3 ›› Issue (1): 45-49.DOI: 10.3969/j.issn.2095-2341.2013.01.09

• 技术与方法 • 上一篇    下一篇

植物化学诱导基因表达系统研究进展

马延娜1,2,薛静2,徐摇光2,张晓东2*   

  1. 1.首都师范大学生命科学院, 北京 100037;
    2.北京市农林科学院北京农业生物技术研究中心, 北京 100097
  • 收稿日期:2012-11-01 出版日期:2013-01-25 发布日期:2012-12-25
  • 通讯作者: 张晓东,研究员,博士,主要从事作物遗传育种研究。E-mail:zhangxiaodong@baafs.net.cn
  • 作者简介:马延娜,硕士研究生,研究方向为分子遗传学。

The Development of Chemical Induced Gene Expression System in Plants

Ma Yan-na, XUE Jing, Xu Yao-guang, ZHANG Xiao-dong   

  1. 1.Collage of Life Science, Capital Normal University, Beijing 100037, China;
    2.Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China
  • Received:2012-11-01 Online:2013-01-25 Published:2012-12-25

摘要: 随着植物基因工程的发展,植物的化学诱导表达系统受到越来越多的重视。化学诱导表达系统通常包含两个元件,第一个元件多为一个嵌合的转录因子,可以特异性的结合到启动子上;第二个元件具有前面的转录因子的结合位点,从而控制目的基因的表达。本文综述了当前广泛应用的一元诱导基因表达系统,如四环素诱导表达系统,和二元诱导表达系统,包括:乙醇诱导、地塞米松诱导、β2雌激素诱导和热休克诱导等不同系统,总结了各化学诱导表达系统的最新研究进展及其潜在利用的可行性。

关键词: 化学诱导, 激活子, 效应因子, 一元基因盒, 二元基因盒

Abstract: With the development of plant genetic engineering, the application of plant chemical induced gene expression system is becoming more and more important. Chemical induced gene expression system typically consists of two components, the first component is mostly a chimeric transcription factor which can specific bind to the promoter. The second element has a transcription factor binding site for the first element, and thereby controlling the expression of target genes. Here, we review the current widely used one component inducible gene expression system, such as the tetracycline inducible expression system, and two components inducible expression systems, including: ethanol-induced, dexamethasone-induced, β2 estrogen hormone-induced and heat shock-induced systems. This paper mainly discusses the recent progress on chemical inducing gene expression systems and their potential use in plants.

Key words: chemical induction, activator, effector, single gene cassette, binary gene cassette