关键词: 抗根肿病, 花药培养, 胚状体, 诱导率

Abstract: Chinese cabbage is one of the important vegetables in northern China. Clubroot disease seriously influenced the production of Chinese cabbage in the world. The use of anther culture can greatly accelerate breeding process of Chinese cabbage  resistant to clubroot. Thirty three Chinese cabbage varieties (lines) resistant to clubroot were used to anther culture in this study. The results indicated that the embryo induction rate of varieties were 72.7%, and the highest rate was 1.86 embryos / bud for Oriental Crown×C11 lines. The medium plus 0.4 g/L glutamine was best for induction of most genotypes. The results also showed that donor plants growing in the greenhouse were easier to induce embryoids. Green cotyledon-type embryos were transferred to solid medium with B5+0.2 mg/L BA+0.1 mg/L NAA+0.1% activated carbon to induce adventitious buds.

Key words: clubroot resistance, anther culture, embryoid, induction rate