关键词: &gamma, -氨基丁酸, 谷氨酸, 发酵液, 膜分离, 离子交换

Abstract: In order to explore the extraction technology of GABA from fermentation broth with glutamic acid as substrate, membrane filtration associated with ion\|exchange adsorption and desorption process were studied in this paper, and followed with decolorization by using active carbon. The initial trans\|membrane pressure is 0.85 bar during the filtration process, and the temperature was controlled at 38℃. With QY-021-a strong acid cation exchange resin as packing in ion\|exchange column, adsorption-desorption process was exerted at room temperature. After concentrated by rotary evaporation, decolorization was performed using 15% (carbon/solids in the solution) activate carbon, with temperature fixed at 65℃, stirring for 40 min. Results showed that the average flux was 128 L/m2·h, and concentration ratio was 33.3. Samplings to detect membrane permeate showed that the yield of GABA was 97.7%, which indicated that there was no GABA retention of interception and adsorption on microfiltration membrane surface and fouling layer. After collecting 5 BV permeate to calculate the yield of ion-exchange process, results showed that the yield was 92.8%, decolorization ratio was 94.2% by using activate carbon B, and the GABA yield was 99.2%. The total yield of the whole process was up to 85%, which indicated that this process has the prospects for industrial application, and further improvements of yield could be achieved by optimized ion exchange processes and equipment.

Key words: GABA, glutamic acid, broth, membrane seperation, ion-exchange