生物技术进展 ›› 2014, Vol. 4 ›› Issue (4): 245-250.DOI: 10.3969/j.issn.2095-2341.2014.04.03

• 进展评述 • 上一篇    下一篇

几种基于PCR的分子标记在甘薯遗传多样性研究中的应用

何虎翼,谭冠宁,何新民*,何海旺,李丽淑,唐洲萍,王晖   

  1. 广西农业科学院经济作物研究所, 南宁 530007
  • 收稿日期:2014-04-24 出版日期:2014-07-25 发布日期:2014-06-10
  • 通讯作者: 何新民,研究员,主要从事甘蔗组培脱毒快繁和选育,以及马铃薯、红薯等作物选育研究。E-mail:he-xinming@163.com
  • 作者简介:何虎翼,助理研究员,主要从事红薯、马铃薯育种和栽培技术研究。E-mail:wingtiger2008@126.com。
  • 基金资助:

    广西自然科学基金项目(2012GXNSFAA053064)资助。

Application of Several PCR-based Molecular Markers in Sweet Potato Genetic Diversity Researches

HE Hu-yi, TAN Guan-ning, HE Xin-min, HE Hai-wang, LI Li-shu, TANG Zhou-ping, WANG Hui   

  1. Cash Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
  • Received:2014-04-24 Online:2014-07-25 Published:2014-06-10

摘要: 遗传多样性是甘薯品种遗传改良的基础。由于分子标记具有数量极大、不受环境及基因表达与否的限制、多为共显性、不影响生物性状表现等优点,现已在甘薯遗传多样性研究中得到广泛应用。本文比较了RAPD、AFLP、SSR、ISSR和SRAP等几种基于PCR的分子标记方法,分别从遗传差异和亲缘聚类分析两方面,对它们在甘薯遗传多样性研究中的应用进行了综述。对比分析表明ISSR是一种共显性、成本较低、重复性好、多态性较高且非常有发展前途的分子标记,并已经被广泛应用到甘薯遗传多样性、物种亲缘关系、系统分类和辅助育种研究中。

关键词: 分子标记, 甘薯, 遗传多样性, 应用

Abstract: Genetic diversity is the basis for genetic improvement of sweet potato varieties. Due to the advantages of molecular markers, such as the great numbers, no restriction of environment and gene expression, mostly co-dominance,  and no effection on biological traits,  molecular markers technologies have been widely used in studies of genetic diversity in sweet potato. This paper compared several PCR\|based molecular markers such as RAPD,  AFLP,  SSR,  ISSR and SRAP. And their applications in the study of sweet potato genetic diversity were reviewed from two aspects,  genetic differences and relationship cluster analysis,  respectively. The comparative analysis indicated that ISSR was a kind of co-dominant and low cost molecular marker with good reproducibility,  high polymorphism, and very promising prospect, which has been widely used in researches of sweet potato including genetic diversity,  species relationship, taxonomy and assisted breeding.

Key words: molecular markers, sweet potato, genetic diversity, application