生物技术进展 ›› 2014, Vol. 4 ›› Issue (1): 40-43.DOI: 10.3969/j.issn.2095-2341.2014.01.08

• 研究论文 • 上一篇    下一篇

胰脂肪酶抑制剂产生菌的筛选

孙菲,郑榕,杨煌建,张祝兰*   

  1. 福建省微生物研究所, 福州 350007
  • 收稿日期:2013-11-29 出版日期:2014-01-25 发布日期:2013-12-24
  • 通讯作者: 张祝兰,研究员,主要从事微生物药物研究。E-mail:jessylan9963@sina.com
  • 作者简介:孙菲,助理研究员,硕士,主要从事微生物药物研究。 E-mail: dearsf999@sohu.com
  • 基金资助:

    福建省自然科学基金项目(2012J01309)资助。

Screening of Pancrelipase Inhibitor\|producing Strains

SUN Fei, ZHENG Rong, YANG Huang-jian, ZHANG Zhu-lan   

  1. Fujian Institute of Microbiology, Fuzhou 350007, China
  • Received:2013-11-29 Online:2014-01-25 Published:2013-12-24

摘要: 本研究采用胰脂肪酶抑制剂筛选模型结合淀粉酶、蛋白酶抑制活性的测定,对来源于土壤的微生物进行高通量筛选,得到三株胰脂肪酶抑制剂产生菌FIM\|1013、FIL\|19和FIMe\|3,其发酵产物对胰脂肪酶活性的抑制率分别为61%、53%和42%,对淀粉酶活性的抑制率分别为0、7%和3%,对蛋白酶活性的抑制率分别为6%、4%和0。结果显示,筛选所得的三个菌株均能产生具有选择性的胰脂肪酶抑制剂,值得进一步的研究。

关键词: 胰脂肪酶, 抑制剂, 筛选模型, 蛋白酶, 淀粉酶

Abstract: A pancreatic lipase inhibitor screening model was constructed, which combined with determination of inhibitory activities of amylase, protease and lipase. Three strains FIM-1013, FIL-19 and FIMe-3 were obtained through target screening of microbial broths from soil organisms by this model. The lipase inhibitory activites of FIM-1013,FIL-19 and FIMe-3 were 61%,53% and 42%, the amylase inhibitory activites were 0,7% and 3%, and the inhibitory protease activity were 6%,4% and 0. The results indicated that the three strains could produce very potent selective inhibitor of pancreatic lipase which deserved further research.

Key words: pancrelipase, inhibitor, screening model, protease, amylase