关键词: 鸡新城疫, 鸡链球菌病, 生物信息学, 共享差异表达基因

Abstract:

In order to explore the common genes and molecular interaction mechanism of Newcastle disease virus and Streptococcus after infection， and to gain a deeper understanding of the synergistic infection relationship between the two， the experiment obtained the relevant gene chip datasets of Newcastle disease virus and Streptococcus clinically infected chickens from the GEO database， and conducted secondary bioinformatics data mining， and carried out GO and KEGG pathway analysis and protein interaction network construction. The results showed that there were 34 differentially expressed genes （DEGs） after Newcastle disease virus infection and Streptococcus enteritidis infection. Through GO functional annotation and KEGG enrichment analysis， the above genes were mainly involved in biological processes such as response to viruses， cell death， programmed cell death， regulation of cytokine production， and NOD-like receptor signaling pathway， cytokine receptor interaction， Toll-like receptor signaling pathway. The results of protein interaction network revealed there were 15 interacting proteins with chicken Newcastle disease virus and Streptococcus infection. Further， 10 pivotal genes， including IL6、TNFSF13B、TNRSF1A、IL1B、TLR4、CD83、IRF7、IRF1、SOCS1、SOCS3， were screened， which were all differentially up-regulated genes. The clinical verification results of core genes showed that some core genes were significantly up-regulated consistently in both Newcastle disease virus infection and Streptococcus infection， which further indicated that there was an interaction between the two. In conclusion， Newcastle disease and Avian Streptococcosis not only behaved similar clinical symptoms， but also had interacting genes in the mechanism of action.

Key words: Newcastle disease in chickens, Avian Streptococcosis, bioinformatics, shared differentially expressed genes