关键词: 启动子, 克隆, 生物信息学, 转录因子, 甲基化, 多态性

Abstract: Promoter is an important element in regulation of gene expression. The regulation role of promoter is the result of a multi-level interaction of multiple factors. The level, location and manner of gene expression are regulated by the promoter, so deeply study of promoter is of great importance for understanding biological growth, defense system and disease. This paper reviews the methods of promoter cloning, bioinformatics analysis and forecasting of promoter, compares two methods of promoter analysis, introduces the progress of promoter methylation and polymorphism, so as to provide a reference for further study of promoters.

Key words: promoter, clone, bioinformatics, transcription factor, methylation, polymorphism