关键词: 棉花, 拮抗菌, 黄萎病, 大丽轮枝菌, 贝莱斯芽孢杆菌

Abstract:

Cotton is an important strategic and livelihood material， which faces many unfavorable factors in the cultivation process， among which cotton Verticillium wilt， as a very harmful plant disease， could cause a large reduction in cotton production. Therefore， a safe and efficient method is needed to deal with the occurrence of cotton Verticillium wilt. We extracted and purified the strain BJB01 from a severely diseased cotton field in Xinjiang. Physiological and biochemical identification and Gram staining test were performed. And we also extracted the DNA of the strain for 16S rDNA sequencing， and blasted the sequencing results on NCBI； then we used the plate standoff test to initially test the effectiveness of the prevention and control； finally， we screened the best prevention and control method and concentration through the greenhouse prevention and control test. Through the test results， the bacterium was found to be 99.71% similar to Bacillus belleiensis， which can be identified as B. belleiensis； and the bacterium showed inhibition of Verticillium dahlia Kleb. in the plate standoff test； through the greenhouse efficacy test， it was found that the bacterium at the concentration of OD₆₀₀=0.6 had the preventive effect of 89.35%， which was better than other methods and treatment concentrations， and the preventive effect of 85.91% was still the best effect in the separate test again. In the comprehensive analysis， Bacillus pelliculatus BJB01 obtained from the cotton disease field in Xinjiang can inhibit Verticillium dahliae， which could be prepared as a biofungicide for cotton production.

Key words: cotton, antagonistic bacteria, verticillium wilt, Verticillium dahlia, Bacillus belleiensis