关键词: 激光捕获显微切割, 肿瘤, 蛋白质组学, 基因组学, 转录组学

Abstract: In today’s society, tumor has become an important disease threatening human health due to its high morbidity and mortality. The research and exploration of its pathogenesis and treatment methods are also deepening. With the development of single-cell multi-omics sequencing technology, the cell heterogeneity of tumor tissues has gradually been recognized by researchers. In order to solve this problem, laser capture microdissection (LCM) technology came into being. LCM technology is a sample collection technology that accurately obtains a specific cell population or individual cells from organs or tissues under the direct visualization of a microscope. LCM technology combined with a variety of molecular biological methods can carry out multi-omics studies on heterogeneous tissues, enriching the existing tumor proteomics, genomics and transcriptomic maps. Therefore, LCM technology has become a powerful tool for studying specific expression and molecular mechanisms, and has been widely used in the field of oncology. Based on this situation, the principle, advantages of LCM and its application in tumor multi-omics study were reviewed, and its possible development direction in the future was prospected, so as to provide new ideas for tumor research and treatment.

Key words: LCM, tumor, proteomics, genomics, transcriptomics